AFLP; Chiken; DNA fingerprinting; authentification; certified productions; slow-growing strains
Abstract :
[en] The European chicken meat market is characterized by numerous quality marks: “Label de Qualité Wallon” in Belgium, “Label Rouge” in France, denominations of geographical origin, organic agriculture, etc. Most of those certified productions have specifications requiring the use of slow-growing chicken strains. The amplified fragment length polymorphism (AFLP) technique has been used to search molecular markers able to discriminate slow-growing chicken strains from fast-growing ones and to authenticate certified products. Two pairs of restriction enzymes (EcoRI/MseI and EcoRI/TaqI) and 121 selective primer combinations were tested on individual DNA samples from chicken products essentially in carcass form that were ascribed as belonging to either slow- or fast-growing strains. Within the resulting fingerprints, two fragments were identified as type−strains specific markers. One primer combination gives a band (333 bp) that is specific for slow-growing chickens, and another primer pair generates a band (372 bp) that was found to be characteristic of fast-growing chickens. The two markers were isolated, cloned, and sequenced. The effectiveness and the specificity of the two interesting determinants were assessed on individuals of two well-known strains (ISA 657 and Cobb 500) and on commercialized products coming from various origins.
The Scientific Support Plan for a Sustainable development Policy-Standards for Food Products of the Belgian Federal office for Scientific, Technical, and Cultural Affairs (OSTC)
Council Regulation (EEC) No. 2081/92, July 14, 1992, on the protection of geographical indications and designations of origin for agricultural products foodstuffs. Off. J. Eur. Communities 1992, L208 (June 24), 1-8.
Fumière, O.; Sinnaeve, G.; Dardenne, P. Attempted authentication of cut pieces of chicken meat from certified production using near-infrared spectroscopy. J. Near Infrared Spectrosc. 2000, 8, 27-34.
Commission Regulation (EEC) No. 1538/91, June 5, 1991, introducing detailed rules for implementing Regulation (EEC) No. 1906/90 on certain marketing standards for poultry. Off. J. Eur. Communities 1991, L143 (June 7), 11-22.
Fumière, O.; Sinnaeve, G.; Dardenne, P. L'authentification rapide des poulets de chair sous label: distinction entre poulets issus de souches à croissance lente ou rapide par la spectrométrie dans le proche infrarouge. Biotechnol. Agron. Soc. Environ. 2000, 4 (4), 214-220.
Vos, P.; Hogers, R.; Bleeker, M.; Reijans, M.; van de Lee, Th.; Hornes, M.; Frijters, A.; Pot, J.; Peleman, J.; Kuiper, M.; Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995, 23, 4407-4414.
Ballvora, A.; Hesselbach, J.; Niewöhner, J.; Leister, D.; Salamini, F.; Gebhardt, C. Marker enrichment and high-resolution map of the segment of potato chromosome VII harbouring the nematode resistance gene GroI. Mol. Gen. Genet. 1995, 249, 82-90.
Meksem, K.; Leister, D.; Peleman, J.; Zabeau, M.; Salamini, F.; Gebhardt, C. A high-resolution map of the vicinity of the RJ locus on chromosome V of potato based on RFLP and AFLP markers. Mol. Gen. Genet. 1995, 249, 74-81.
Thomas, C. M.; Vos, P.; Zabeau, M.; Jones, D. A.; Norcott, K. A.; Chadwick, B. P.; Jones, J. D. G. Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to tomato Cf-9 gene for resistance to Cladosporium fulvum. Plant J. 1995, 8, 785-794.
Becker, J.; Vos, P.; Kuiper, M.; Salamini, F.; Heun, M. Combined mapping of AFLP and RFLP markers in barley. Mol. Gen. Genet. 1995, 249, 65-73.
Mano, Y.; Kawasaki, S.; Takaiwa, F.; Komatsuda, T. Construction of a genetic map of barley (Hordeum vulgare L.) cross 'Azumamugi' x 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system. Genome 2001, 44, 284-292.
Mackill, D. J.; Zhang, Z.; Redofia, E. D.; Colowit, P. M. Level of polymorphism and genetic mapping of AFLP markers in rice. Genome 1996, 39, 969-977.
Gedil, M. A.; Wye, C.; Berry, S.; Segers, B.; Peleman, J.; Jones, R.; Leon, A.; Slabaugh, M. B.; Knapp, S. J. An integrated restriction fragment length polymorphism-amplified fragment length polymorphism linkage map for cultivated sunflower. Genome 2001, 44, 213-221.
Quagliaro, G.; Vischi, M.; Tyrka, M.; Olivieri, AM. Identification of wild and cultivated sunflower for breeding purposes by AFLP markers. J. Heredity 2001, 92, 38-42.
Otsen, M.; den Bieman, M.; Kuiper, M. T. R.; Pravenec, M.; Kren, V.; Kurtz Th, W.; Jacob H. J.; Lankhorst, Æ.; van Zutphen, B. F. M. Use of AFLP markers for gene mapping and QTL detection in the rat. Genomics 1996, 37, 289-294.
Ajmone-Marsan, P.; Valentini, A.; Cassandro, M.; Vecchiotti-Antaldi, G.; Bertoni, G.; Kuiper, M. AFLP markers for DNA fingerprinting in cattle. Anim. Genet. 1997, 28, 418-426.
Plastow, G. S.; Kuiper, M.; Wales, R.; Archibald, A. L.; Haley, C. S.; Siggens K. W. AFLP for mapping and QTL detection in commercial pigs. Proceedings of the 6th World Congress on Genetics Applied to Livestock Production, The University of New England: Armidale, Australia; 1998; Vol. 26, pp 209-212.
Liu, Z.; Nichols, A.; Li, P.; Dunham, R. A. Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2, and backcross hybrids. Mol. Gen. Genet. 1998, 258, 260-268.
Groenen, M. A. M.; Crooijmans, R. P. M. A.; Veenendaal, A.; Cheng, H. H.; Siwek, M.; Van der Poel, J. J. A comprehensive microsatellite linkage map of the chicken genome. Genomics 1998, 49, 265-274.
Griffiths, R.; Orr, K. The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers. Mol. Ecol. 1999, 8, 671-674.
Óvilo, C.; Cervera, M. T.; Castellanos, C.; Martínez-Zapater, J. M. Characterisation of Iberian pig genotypes using AFLP markers. Anim. Genet. 2000, 31, 117-122.
Ajmone-Marsan, P.; Negrini, R.; Crepaldi, P.; Milanesi, E.; Gorni, C.; Valentini, A.; Cicogna, M. Assessing genetic diversity in Italian goat populations using AFLP(R) markers. Anim. Genet. 2001, 32, 281-288.
Crepaldi, P.; Negrini, R.; Milanesi, E.; Gomi, C.; Cicogna, M.; Ajmone-Marsan P. Diversity in five goat populations of the Lombardy Alps: comparison of estimates obtained from morphometric traits and molecular markers. J. Anim. Breed. Genet. 2001, 118, 173-180.
Alves, E.; Castellanos, C.; Ovilo, C.; Silió, L.; Rodrígez, C. Differentiation of the raw material of the Iberian pig meat industry based on the used of amplified fragment length polymorphism. Meat Sci. 2002, 61, 157-162.
Sambrook, J.; Fritsch, E. F.; Maniatis, T. In Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
Herbergs, J.; Siwek, M.; Crooijmans, R. P. M. A.; Van der Poel, J. J.; Groenen, M. A. M. Multicolour fluorescent detection and mapping of AFLP markers in chicken (Gallus domesticus). Anim. Genet. 1999, 30, 274-285.
Knorr, C.; Cheng, H. H.; Dodgson, J. B. Application of AFLP markers to genome mapping in poultry. Anim. Genet. 1999, 30, 28-35.
van der Voort, J. N. R.; van Zandvoort, P.; van Eck, H. J.; Folkertsma, R. T.; Hutten, R. C. B.; Draaistra, J.; Gommers, F. J.; Jacobsen, E.; Helder, J.; Bakker, J. Use of allele specificity of comigrating AFLP markers to align genetic maps from different potato genotypes. Mol. Gen. Genet. 1997, 255, 438-447.
Meksem, K.; Ruben, E.; Hyten, D.; Triwitayakom, K.; Lightfoot, D. A. Conversion of AFLP bands into high-throughput DNA markers. Mol. Genet. Genomics 2001, 265, 207-21.
Paran, Y.; Michelmore, R. W. Development of reliable PCR- based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 1993, 85, 985-993.
Theuns, I.; Windels, P.; De Buck, S.; Depicker, A.; Van Bockstaele, E.; De Loose, M. Identification and characterization of T-DNA inserts by T-DNA fingerprinting. Euphytica 2002, 123, 75-84.
