Scientific conference in universities or research centers (Scientific conferences in universities or research centers)
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS (powerpoint)
[en] We present here a rapid, easy, reliable and cost-effective method for the quantification of 25-hydryxoyvitamin D2 and D3, epi-25-hydroxyyvitamin D3 and 24,25-dihydroxyvitamin D3 by LC-MS/MS in serum samples. The proposed methodology has been strongly validated with both NIST and Labquality materials, obtaining mean intra-assay and inter-assay imprecision lower than 6 and 6.4% and mean recoveries within 95-104%. Besides we have compared satisfactorily samples from Vitamin D Standardization Program (n=80) with reference values and patient samples (n=281) with our reference LC-MS/MS method.
The proposed methodology is prepared to be used in routine testing and permits the calculation of the Vitamin D Metabolite Ratio (VMR).
Disciplines :
Endocrinology, metabolism & nutrition
Author, co-author :
Fabregat Cabello, Neus ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Huyghebaert, Loreen ; Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
PEETERS, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
LE GOFF, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
Cavalier, Etienne ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Language :
English
Title :
A fast and simple method for simultaneous measurements of 25(OH)D, 24,25(OH)2D and the Vitamin D Metabolite Ratio (VMR) in serum samples by LC-MS/MS (powerpoint)
