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Abstract :
[en] Erythritol (1,2,3,4-butanetetrol) is a four-carbon sugar alcohol used as sweeteners in food and beverage industry. It is most commonly generated from glucose via fermentation using osmophilic yeasts. Yarrowia lipolytica is highly proficient at producing erythritol and can use raw glycerol instead of glucose as its main carbon source. Raw glycerol is a byproduct of the biodiesel industry, and it is a cheaper and more efficient carbon source than glucose when it comes to producing erythritol. To date, most studies seeking to improve erythritol production have used wild type strains or randomly generated mutants and have focused on optimizing the culture medium or culturing conditions. In this study, we demonstrated that metabolic engineering can be used to enhance the production of erythritol from glycerol. To this end, we constructed a set of eleven strains that overexpressed genes encoding key enzymes of either glycerol catabolism and erythritol synthesis pathway. For the best of our engineered strains, erythritol productivity in bioreactor was increased 78% relative to the wild type strain and maximum concentrations were obtained in 40% less time.