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Enhancing the sweetener erythritol productivity in Yarrowia lipolytica using metabolic engineering
Carly, Frédéric; Vandermies, Marie; Nicaud, Jean-Marc et al.
201733rd International Specialised Symposium on Yeast
 

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Keywords :
Yarrowia lipolytica; Erythritol; Metabolic engineering; Glycerol kinase; Transketolase; Erythrulose kinase
Abstract :
[en] Erythritol (1,2,3,4-butanetetrol) is a four-carbon sugar alcohol used as sweeteners in food and beverage industry. It is most commonly generated from glucose via fermentation using osmophilic yeasts. Yarrowia lipolytica is highly proficient at producing erythritol and can use raw glycerol instead of glucose as its main carbon source. Raw glycerol is a byproduct of the biodiesel industry, and it is a cheaper and more efficient carbon source than glucose when it comes to producing erythritol. To date, most studies seeking to improve erythritol production have used wild type strains or randomly generated mutants and have focused on optimizing the culture medium or culturing conditions. In this study, we demonstrated that metabolic engineering can be used to enhance the production of erythritol from glycerol. To this end, we constructed a set of eleven strains that overexpressed genes encoding key enzymes of either glycerol catabolism and erythritol synthesis pathway. For the best of our engineered strains, erythritol productivity in bioreactor was increased 78% relative to the wild type strain and maximum concentrations were obtained in 40% less time.
Disciplines :
Biotechnology
Author, co-author :
Carly, Frédéric
Vandermies, Marie ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Nicaud, Jean-Marc
Fickers, Patrick ;  Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Language :
English
Title :
Enhancing the sweetener erythritol productivity in Yarrowia lipolytica using metabolic engineering
Publication date :
June 2017
Event name :
33rd International Specialised Symposium on Yeast
Event organizer :
Microbiology Society
Event place :
Cork, Ireland
Event date :
from June 26th to 29th
Audience :
International
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 28 August 2017

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