[en] Erythritol is a four-carbon sugar alcohol synthesized by osmophilic yeasts, such as Yarrowia lipolytica, in response to osmotic stress. This metabolite has application as food additive due to its sweetening properties. Although Y. lipolytica can produce erythritol at a high level from glycerol, it is also able to consume it as carbon source. This ability negatively affects erythritol productivity and represents a serious drawback for the development of an efficient erythritol production process. In this study, we have isolated by insertion mutagenesis a Y. lipolytica mutant unable to grow on erythritol. Genomic characterization of the latter highlighted that the mutant phenotype is directly related to the disruption of the YALI0F01606g gene. Several experimental evidences suggested that the identified gene, renamed EYK1, encodes an erythrulose kinase. The mutant strain showed an enhanced capacity to produce erythritol as compared to the wild-type strain. Moreover, in specific experimental conditions, it is also able to convert erythritol to erythrulose, another compound of biotechnological interest.
Disciplines :
Biotechnology
Author, co-author :
Carly, Frédéric
Gamboa-Melendez, Heber
Vandermies, Marie ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Damblon, Christian ; Université de Liège > Département de chimie (sciences) > Chimie biologique structurale
Nicaud, Jean-Marc
Fickers, Patrick ; Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies
Language :
English
Title :
Identification and characterization of EYK1, a key gene for erythritol catabolism in Yarrowia lipolytica
Publication date :
2017
Journal title :
Applied Microbiology and Biotechnology
ISSN :
0175-7598
eISSN :
1432-0614
Publisher :
Springer Verlag, Berlin, Germany
Volume :
101
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
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