The miR-183/ItgA3 axis is a key regulator of prosensory area during early inner ear development

[en] MicroRNAs are important regulators of gene expression and are involved in cellular processes such as proliferation or differentiation, particularly during development of numerous organs including the inner ear. However, it remains unknown if miRNAs are required during the earliest stages of otocyst and cochlear duct development. Here, we report that a conditional loss of Dicer expression in the otocyst impairs the early development of the inner ear as a result of the accumulation of DNA damage that trigger p53-mediated apoptosis. Moreover, cochlear progenitors in the prosensory domain do not exit the cell cycle. Our unbiased approach identified ItgA3 as a target of miR-183, which are both enriched in the otic vesicle. We observed that the repression of integrin alpha 3 by miR-183 controls cell proliferation in the developing cochlea. Collectively, our results reveal that Dicer and miRNAs play essential roles in the regulation of early inner ear development.Cell Death and Differentiation advance online publication, 4 August 2017; doi:10.1038/cdd.2017.127.

Research center :

Giga-Neurosciences - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Van Den Ackerveken, Priscilla ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Mounier, Anaïs ; Université de Liège > GIGA - Neurosciences

Huyghe, Aurélia

SACHELI, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > Service de microbiologie clinique

Van Lerberghe, Pierre-Bernard ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Volvert, Marie-Laure

Delacroix, Laurence ; Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques

Nguyen, Laurent ; Université de Liège > GIGA - Neurosciences

Malgrange, Brigitte ; Université de Liège > GIGA - Neurosciences

Language :

English

Title :

The miR-183/ItgA3 axis is a key regulator of prosensory area during early inner ear development

Publication date :

2017

Journal title :

Cell Death and Differentiation

ISSN :

1350-9047

eISSN :

1476-5403

Publisher :

Nature Publishing Group, London, United Kingdom

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
Fonds Léon Fredericq [BE]
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]

Available on ORBi :

since 03 July 2017

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Bibliography

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