Article (Scientific journals)
From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
Ceusters, Justine; Lejeune, Jean-Philippe; Sandersen, Charlotte et al.
2017In Scientific Reports, 7 (696)
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Keywords :
Mesenchymal stem cells; orthopaedics
Abstract :
[en] Bone marrow and adipose tissue represent the two most commonly exploited sources of adult mesenchymal stem cells for musculoskeletal applications. Unfortunately the sampling of bone marrow and fat tissue is invasive and does not always lead to a sufficient number of cells. The present study describes a novel sampling method based on microbiopsy of skeletal muscle in man, pigs, dogs and horses. The process includes explant of the sample, Percoll density gradient for isolation and subsequent culture of the cells. We further characterized the cells and identified their clonogenic and immunomodulatory capacities, their immune-phenotyping behavior and their capability to differentiate into chondroblasts, osteoblasts and adipocytes. In conclusion, this report describes a novel and easy-to-use technique of skeletal muscle-derived mesenchymal stem cell harvest, culture, characterization. This technique is transposable to a multitude of different animal species.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ceusters, Justine ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Chirurgie et clinique chirurgicale des petits animaux
Lejeune, Jean-Philippe ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Pathologie médicale des petits animaux
Sandersen, Charlotte  ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie et réanimation vétérinaires
Lagneaux, Laurence;  Université Libre de Bruxelles - ULB > Clinical cell therapy
Serteyn, Didier  ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
From skeletal muscle to stem cells: an innovative and minimally-invasive process for multiple species
Publication date :
2017
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
7
Issue :
696
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 August 2017

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