Reference : Validation of a new pregnancy-associated glycoprotein radioimmunoassay method for the de...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/21310
Validation of a new pregnancy-associated glycoprotein radioimmunoassay method for the detection of early pregnancy in ewes.
English
Barbato, Olimpia [University of Perugia Italy > > > > > >]
Melo de Sousa, Noelita mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Debenedetti, A. [University of Perugia Italy > > > > > >]
Canali, C. [University of Perugia Italy > > > > > >]
Todini, L. [University of Camerino Italy > > > > > >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
2009
Theriogenology
Elsevier
72
993–1000
Yes (verified by ORBi)
International
0093-691X
New York
NY
[en] Early pregnancy ; Pregnancy-associated glycoprotein ; Progesterone ; Radioimmunoassay ; Sarda ewe
[en] The aim of the current study was to describe the use of a pool of different antisera raised against pregnancy-associated glycoproteins (PAGs; purified from both ovine and caprine placentas) for early pregnancy diagnosis in ovine species. Sixty-three pluriparous Sarda ewes (Ovis aries) were synchronized. Blood samples were withdrawn on Days 18, 24, 26, 28, 30, and 50 after mating. These samples were assayed for progesterone (radioimmunoassay [RIA] including an extraction step) and for pregnancy-associated glycoproteins (RIA-706 and RIA-srPool). Progesterone concentrations were under 1.0ng/mL in all nonpregnant Sarda ewes. In pregnant ewes, mean progesterone concentrations ranged from 2.4ng/mL (Day 24, single pregnancies) to 4.4ng/mL (Day 28, multiple pregnancies). During all periods of examination, PAGs remained lower than 0.8ng/mL in nonpregnant ewes. On Day 18 of pregnancy, PAG concentrations could be detected in 26 of 43 (60.5%) and in 41 of 43 (95.3%) pregnant ewes using the RIA-706 and RIA-srPool methods, respectively. From Day 24 to Day 50, using both RIA methods, PAGs could be detected in all pregnant ewes. On Day 24, the best threshold for pregnancy diagnosis was obtained by use of RIA-srPool, maximal concentration in nonpregnant ewes being 0.3ng/mL and minimal concentration in pregnant ewes being 4.8ng/mL. In general, progesterone and PAG concentrations were higher in multiple pregnancies than in single pregnancies. However, because of large individual variations, single pregnancies could not be differentiated from multiple pregnancies
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/21310
10.1016/j.theriogenology.2009.06.020

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