Reference : EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing ...
Scientific journals : Article
Life sciences : Biotechnology
http://hdl.handle.net/2268/212994
EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica
English
Vandermies, Marie mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
Denies, Olivia mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
Nicaud, Jean-Marc []
Fickers, Patrick mailto [Université de Liège > Agronomie, Bio-ingénierie et Chimie (AgroBioChem) > Microbial, food and biobased technologies >]
Aug-2017
Journal of Microbiological Methods
Elsevier Science
139
161-164
Yes (verified by ORBi)
International
0167-7012
Amsterdam
The Netherlands
[en] Yarrowia lipolytica ; Genome editing ; EYK1 ; Catabolic selectable marker
[en] We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA
http://hdl.handle.net/2268/212994
10.1016/j.mimet.2017.05.012

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