Poster (Scientific congresses and symposiums)
A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Farhat, Khalil; Bodart, Gwennaëlle; Renard, chantal et al.
2017The 19th European Congress of Endocrinology.
 

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Keywords :
Growth hormone; Infectious diseases; S.pneumoniae
Abstract :
[en] In the framework of close interactions between the immune and neuroendocrine systems, Growth Hormone (GH) has been proposed to exert significant effects on the immune system, but there is not yet a consensus about GH immunomodulatory properties. These studies investigated the immune and anti-infectious response of dwarf Ghrh-/- mice presenting a severe deficiency of the GHRH/GH/IGF-1 axis. In basal conditions, thymic parameters and T-cell responses of Ghrh-/- mice were not severely affected but a constant B-cell lymphopaenia was observed. Thus, we investigated vaccine and anti-infectious responses of Ghrh-/- mice toward Streptococcus pneumonia, a B-dependent pathogen, Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. These vaccines both include the serotype 1 (our S.pneumoniae strain) and provide an effective protection in mice. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH could exert distinct impacts upon spenic areas. Furthermore, after intranasal instillation of a non-lethal dose (defined by the full clearance by WT C57BL/6 mice after 24h) of serotype 1 S.pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility. This was proved by a marked time-dependent increase in pulmonary bacterial, a septicemia already 24h after infection and a survival limit of 72h. We also observed a dramatic decrease in lung B- and T-cell populations and an increase in proportion of inflammatory macrophages. By contrast, wild-type and heterozygote mice completely cleared S.pneumoniae infection after 24h. In conclusion, our data show without ambiguity that the somatotrope GHRH/GH/IGF-1 axis plays an important and unsuspected role in defense against S.Pneumoniae.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Immunology & infectious disease
Author, co-author :
Farhat, Khalil ;  Université de Liège - ULiège > Form. doc. sc. bioméd. & pharma.
Bodart, Gwennaëlle ;  Université de Liège - ULiège > Form. doc. sc. bioméd. & pharma.
Renard, chantal
Desmet, Christophe  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
MOUTSCHEN, Michel  ;  Centre Hospitalier Universitaire de Liège - CHU > Service des maladies infectieuses - médecine interne
BARON, Frédéric  ;  Centre Hospitalier Universitaire de Liège - CHU > Service d'hématologie clinique
Beguin, Yves  ;  Université de Liège > GIGA-R : Hématologie
Salvatori, Roberto;  Johns Hopkins University > Endocrinology
Martens, Henri ;  Université de Liège > Centre d'immunologie
GEENEN, Vincent ;  Centre Hospitalier Universitaire de Liège - CHU > Centre d'immunologie > Service d'endocrinologie clinique
Language :
English
Title :
A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Publication date :
May 2017
Number of pages :
A0
Event name :
The 19th European Congress of Endocrinology.
Event organizer :
The European Society of Endocrinology
Event place :
Lisbon, Portugal
Event date :
20/05/2017 - 23/05/2017
Audience :
International
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Research Concerted Action Somatosthym of the Brussels-Wallonia Federation
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since 16 July 2017

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