Article (Scientific journals)
N-Glycosylation of an IgG antibody secreted by Nicotiana tabacum BY-2 cells can be modulated through co-expression of human β-1,4-galactosyltransferase
Navarre, Catherine; Smargiasso, Nicolas; Duvivier, Laurent et al.
2017In Transgenic Research, 26 (3), p. 375-384
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Keywords :
Antibody; N-Glycosylation; Plant suspension cells; β-1,4-Galactosyltransferase
Abstract :
[en] Nicotiana tabacum BY-2 suspension cells have several advantages that make them suitable for the production of full-size monoclonal antibodies which can be purified directly from the culture medium. Carbohydrate characterization of an antibody (Lo-BM2) expressed in N. tabacum BY-2 cells showed that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (>70%) of the complex GnGnXF glycoform. The stable co-expression of a human β-1,4-galactosyltransferase targeted to different Golgi sub-compartments altered Lo-BM2N-glycosylation and resulted in the production of an antibody that exhibited either hybrid structures containing a low abundance of the plant epitopes (α-1,3-fucose and β-1,2-xylose), or a large amount of galactose-extended N-glycan structures. These results demonstrate the suitability of stable N-glycoengineered N. tabacum BY-2 cell lines for the production of human-like antibodies. © 2017, Springer International Publishing Switzerland.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Navarre, Catherine ;  Université Catholique de Louvain - UCL > Institut des Sciences de la Vie
Smargiasso, Nicolas  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Duvivier, Laurent;  Université Catholique de Louvain - UCL > Institut des Sciences de la Vie
Nader, Joseph;  Université Catholique de Louvain - UCL > Institut des Sciences de la Vie
Far, Johann  ;  Université de Liège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART)
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Boutry, Marc;  Université Catholique de Louvain - UCL > Institut des Sciences de la Vie
 These authors have contributed equally to this work.
Language :
English
Title :
N-Glycosylation of an IgG antibody secreted by Nicotiana tabacum BY-2 cells can be modulated through co-expression of human β-1,4-galactosyltransferase
Publication date :
June 2017
Journal title :
Transgenic Research
ISSN :
0962-8819
eISSN :
1573-9368
Publisher :
Kluwer Academic Publishers, Dordrecht, Netherlands
Volume :
26
Issue :
3
Pages :
375-384
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 July 2017

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