Paper published in a journal (Scientific congresses and symposiums)
Potential use of estetrol in sensorineural hearing loss protection
DELHEZ, Amandine; Manfroid, Isabelle; LEFEBVRE, Philippe et al.
2017In B-ENT
Peer Reviewed verified by ORBi
 

Files


Full Text
170129-Abstract AD.pdf
Author preprint (87.95 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Introduction and Aims: Estrogens have a positive impact on the auditory function. Indeed, estradiol (E2) treatment in animals protects against acoustic trauma and aminoglycoside toxicity and its depletion can contribute to age-related hearing loss. However, estradiol is not suitable in clinical practice, given the increased risk of cancer and thromboembolic events. There is a need to find new treatments that present a higher benefit/risk ratio. In this work, we studied a new natural estrogen derivative, estetrol (E4), that was shown to act as a Selective Estrogen Receptor Modulator (SERM). Estetrol is thus able to display estrogen-like as well as antiestrogen activity depending on the target tissue. Interestingly, E4 allows the prevention of menopause symptoms such as osteoporosis or hot flushes through an agonistic action, whereas it exerts anti-estrogenic effects on the mammary gland. These preclinical data suggest that E4 presents a safer profile than E2. Material and Methods: To investigate whether this molecule presents otoprotective actions, we exposed P2 cultured organs of Corti to gentamicin, with or without estetrol (E4). After 48 hours of culture, hair cell survival was evaluated following anti-myosin VII Immunohistochemistry. We next moved on to in vivo studies using young zebrafish larvae (5dpf), since they constitute a highly effective model to determine the potential ototoxicity of drugs and to screen for candidates of hair cell protective agents. We monitored the survival of hair cells within neuromasts following chronic or acute exposure to gentamicin. Results: In cochlear culture, gentamicin-induced hair cell loss was significantly reduced by E4. Interestingly, the toxicity of aminoglycoside was also reduced in vivo upon treatment of zebrafish larvae with E4. Conclusion: The survival rate of hair cells was increased in the presence of estetrol both in vitro and in vivo indicating that estetrol constitutes an interesting protective drug against hearing loss.
Disciplines :
Otolaryngology
Author, co-author :
DELHEZ, Amandine ;  Centre Hospitalier Universitaire de Liège - CHU > Service d'ORL, d'audiophonologie et de chir. cervico-faciale
Manfroid, Isabelle ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
LEFEBVRE, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > Service d'ORL, d'audiophonologie et de chir. cervico-faciale
Delacroix, Laurence ;  Université de Liège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Malgrange, Brigitte  ;  Université de Liège > GIGA - Neurosciences
Language :
English
Title :
Potential use of estetrol in sensorineural hearing loss protection
Publication date :
2017
Event name :
Annual Meeting of the Royal Belgian Society for Ear, Nose and Throat, Head and Neck Surgery
Event date :
25/03/2015
Journal title :
B-ENT
ISSN :
1781-782X
eISSN :
2684-4907
Publisher :
Royal Belgian Society, Leuven, Belgium
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 June 2017

Statistics


Number of views
174 (38 by ULiège)
Number of downloads
5 (5 by ULiège)

Bibliography


Similar publications



Contact ORBi