[en] Facing an improvement of the quality assurance in all the laboratory, the validation of methods has become a step that cannot be got round. The objective of this work is the validation of a Protein-Precipitation HPLC-MS/MS method for the measurement of steroid hormones (Aldosterone, Androstenedione, Cortisol, 17-OH Progesterone, DHEAS and Testosterone). To achieve this purpose, a precision, recovery, linearity, carry over assay and a limit of quantification are performed. After collection of the data, results are calculated and statistically analysed. They allow to set boundaries to the method and to understand more easily how we could improve it. The results show that the method is effective for all hormones except the aldosterone, for which the method still has to be improved. Moreover, a comparison of the analysis of the testosterone between Cobas and HPLC MS/MS is made. In the lab, this hormone is analysed with Cobas but a transition to the HPLC MS/MS can be done because the results are satisfactory.
Disciplines :
Laboratory medicine & medical technology
Author, co-author :
Rademaker, Gilles ; Université de Liège > Département des sciences biomédicales et précliniques > GIGA-R : Labo de recherche sur les métastases
Language :
English
Title :
Validation of a Multiplex HPLC MS/MS assay for the measurement of steroid hormones
Defense date :
07 January 2016
Number of pages :
32
Institution :
ULiège - Université de Liège
Degree :
Master en sciences pharmaceutiques, à finalité approfondie
Promotor :
CAVALIER, Etienne ; Centre Hospitalier Universitaire de Liège - CHU > Service de chimie clinique
Name of the research project :
Validation of a multiplex HPLC-MS/MS assay for the measurement of steroid hormones
