Article (Scientific journals)
Reduction of the transcription level of the mitochondrial genome in human glioblastoma.
Dmitrenko, Vladimir; Shostak, Kateryna; Boyko, Oxana et al.
2005In Cancer Letters, 218 (1), p. 99-107
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Keywords :
Animals; Brain Neoplasms/genetics; Cattle; DNA Probes; Electron Transport Complex IV/biosynthesis/genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Library; Glioblastoma/genetics; Humans; Hybridization, Genetic; Mitochondria/genetics; Transcription, Genetic; Tumor Cells, Cultured
Abstract :
[en] Screening of human fetal brain cDNA library by glioblastoma (GB) and normal human brain total cDNA probes revealed 80 differentially hybridized clones. Hybridization of the DNA from selected clones and the same cDNA probes confirmed this difference for 38 clones, of which eight clones contained Alu-repeat inserts with increased levels in GB. Thirty clones contained cDNAs corresponding to mitochondrial genes for ATP synthase subunit 6 (ATP6), cytochrome c oxidase subunit II (COXII), cytochrome c oxidase subunit III (COXIII), NADH dehydrogenase subunit 1 (ND1), NADH dehydrogenase subunit 4 (ND4), and mitochondrial 12S rRNA. The levels of all these mitochondrial transcripts were decreased in glioblastomas as compared to tumor-adjacent histologically normal brain. Earlier we found the same for cytochrome c oxidase subunit I (COXI) Serial Analysis of Gene Expression (SAGE) showed lower content of the tags for all mitochondrial genes in GB SAGE libraries and together with our experimental data could serve as evidence of general inactivation of the mitochondrial genome in glioblastoma--the most malignant and abundant form of human brain tumor.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dmitrenko, Vladimir
Shostak, Kateryna ;  Université de Liège > Département de pharmacie > Chimie médicale
Boyko, Oxana
Khomenko, Olexiy
Rozumenko, Vladimir
Malisheva, Tatiana
Shamayev, Mikhail
Zozulya, Yuriy
Kavsan, Vadym
Language :
English
Title :
Reduction of the transcription level of the mitochondrial genome in human glioblastoma.
Publication date :
2005
Journal title :
Cancer Letters
ISSN :
0304-3835
eISSN :
1872-7980
Publisher :
Elsevier, Netherlands
Volume :
218
Issue :
1
Pages :
99-107
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 June 2017

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