[en] BACKGROUND AND OBJECTIVES: Our objective was to identify differentially expressed genes involved in the pathogenesis of glioblastoma multiforme (GBM). METHODS: Screening of arrayed human fetal brain and human postnatal brain cDNA libraries was performed by differential hybridization with glioblastoma multiforme and human normal brain cDNAs. RESULTS: Repeated differential hybridization of more than 100 cDNA clones selected by primary screening and analysis of RNA from adult normal brain and glial tumors showed 16 nucleotide sequences differentially expressed between normal brain and brain tumors. Among others, decreased content in astrocytic tumors was determined for TSC-22 mRNA corresponding to cDNA in the ICRFp507J1041 clone from human fetal brain cDNA library. Northern blot hybridization of RNA from different human brain tumors showed very low amounts of TSC-22 mRNA in most investigated samples of GBM, anaplastic astrocytoma, and some other tumors. Complete lack of expression of TSC-22 occurred in one sample of anaplastic astrocytoma, as well as in meningioma, brain sarcoma, sarcomatous meningioma, and oligodendroglioma. The differential expression of TSC-22 gene was confirmed by semiquantitative RT-PCR in 15 samples of astrocytomas WHO grade II-IV and three samples of normal brain. CONCLUSIONS: Significantly decreased levels of TSC-22 mRNA in human brain and salivary gland tumors and antiproliferative role of TSC-22 strongly suggest a tumor suppressor role for TSC-22. J.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Shostak, Kateryna ; Université de Liège > Département de pharmacie > Chimie médicale
Dmitrenko, Vladimir V.
Garifulin, Oleg M.
Rozumenko, Vladimir D.
Khomenko, Olexiy V.
Zozulya, Yuriy A.
Zehetner, Gunther
Kavsan, Vadym M.
Language :
English
Title :
Downregulation of putative tumor suppressor gene TSC-22 in human brain tumors.
Publication date :
2003
Journal title :
Journal of Surgical Oncology
ISSN :
0022-4790
eISSN :
1096-9098
Publisher :
John Wiley & Sons, Hoboken, United States - New York
Maier D, Zhang Z, Taylor E, et al.: Somatic deletion mapping on chromosome 10 and sequence analysis of PTEN/MMAC1 point to the 10q25-26 region as the primary target in low-grade and high-grade gliomas. Oncogene 1998;16:3331-3335.
Rosenberg EJ, Lisle DK, Burwick JA, et al.: Refined deletion mapping of the chromosome 19q glioma tumor suppressor gene to the D19S412-STD interval. Oncogene 1996;13:2483-2485.
Ino Y. Silver JS, Blazejewski L, et al.: Common regions of deletions on chromosome 22q12, 22q13.1 and 22q13.2 in human astrocytomas appear related to malignancy grade. J Neuropathol Exp Neurol 1999;58:881-885.
Simons A, Jeuken JW, Eleweld MJ, et al.: Isolation and characterization of glioblastoma-associated homozygously deleted DNA fragments from chromosomal region 9p21 suggests involvement of multiple tumor suppressor genes. J Pathol 1999;189:402-409.
Smith JS, Tachibana I, Allen C, et al.: Cloning of a human ortholog (RPH3AL) of (RNO)Rph3al from a candidate 17p13.3 medulloblastoma tumor suppressor locus. Genomics 1999;59:97-101.
Farrell WE, Clayton RN: Tumor suppressor genes in pituitary tumor formation. Baillieres Best Pract Res Clin Endocrinol Metab 1999;13:81-93.
Bello MJ, de Campos JM, Vaquero J, et al.: High-resolution analysis of chromosome arm lp alterations in meningioma. Cancer Genet Cytogenet 2000;20:30-36.
Shibanuma M, Kuroki T, Nose K: Isolation of a gene encoding a putative leucine zipper structure that is induced by transforming growth factor beta l and other growth factors. J Biol Chem 1992;267:10219-10224.
Dmitrenko VV, Garifulin OM, Smikodub OI, et al.: Analysis of human genome expression by using of cDNA libraries of different tissues. Cytol Genet (Ukr) 1995;29:64-71.
Dmitrenko VV, Garifulin OM, Shostak KO, et al.: Characterization of different types of mRNAs expressing in human brain. Cytol Genet (Ukr) 1996;30:41-47.
Dmitrenko VV, Shostak KO, Garifulin OM, et al.: Changes of gene expression in human brain astrocytomas. Exp Oncol (Ukr) 1998;20:191-197.
Sambrook J, Fritch EF, Maniatis T: "Molecular Cloning: A Laboratory Manual." 2nd Ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 1989.
Chomczynski P, Sacchi N: Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-159.
Rae FK, Stephenson SA, Nicol DL, Clements JA: Novel association of a diverse range of genes with renal cell carcinoma as identified by differential display. Int J Cancer 2000;88:726-732.
Sanger FS. Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977;74:6463-6467.
Diatchenko L, Lukyanov S, Lau YF, et al.: Suppression subtractive hybridization: A versatile method for identifying differentially expressed genes. Methods Enzymol 1999;303:349-380.
Nguyen C, Rocha D, Granjeaud S. et al.: Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones. Genomics 1995;29:207-216.
Jay P, Ji JW, Marsollier C, et al.: Cloning of the human homologue of the TGFbeta-stimulated clone 22 gene. Biochem Biophys Res Commun 1996;222:821-826.
Ohta S, Shimekake Y, Nagata K: Molecular cloning and characterization of a transcription factor for the C-type natriuretic peptide gene promoter. Eur J Biochem 1996;242:460-466.
Dmitrenko VV, Kavsan VM: Variability of polyadenylation sites in mRNAs from human fetal liver. FEBS Lett 1991;280:284-286.
Kestler HA, van der Leebe B-JM, van der Saag PT, van der Burg B: Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation. J Biol Chem 1997;272:16637-16643.
Kestler HA, Blanchetot C, den Hertog J, et al.: Transforming growth factor beta stimulated clone TSC-22 is a member of a family of leucine zipper proteins that can homo- and heterodimerize and has transcriptional repressor activity. J Biol Chem 1999;274:27439-27447.
Riechmann V, van Cruchten I. Sablitzky F: The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3. Nucleic Acids Res 1994;22:749-755.
Nakashiro K, Kawamata H, Hino S, et al.: Down-regulation of TSC-22 (transforming growth factor beta-stimulated clone 22) markedly enhances the growth of a human salivary gland cancer cell line in vitro and in vivo. Cancer Res 1998;58:549-555.
Ohta S, Yanagihara K, Nagata K: Mechanism of apoptotic cell death of human gastric carcinoma cells mediated by transforming growth factor beta. Biochem J 1997;324:777-782.
Kawamata H, Nakashiro K, Uchida D, et al.: Induction of TSC-22 by treatment with a new anti-cancer drug, vesnarinone, in a human salivary gland cancer cell. Br J Cancer 1998;77:71-78.
Henson JW, Schnitker BL, Correa KM, et al.: The retinoblastoma gene is involved in malignant progression of astrocytomas. Ann Neurol 1994;36:714-721.
Venkatraj VS, Begemann M, Sobrino A, et al.: Genomic changes in glioblastoma cell lines detected by comparative genomic hybridization. J Neurooncol 1998;36:141-148.
Harada K, Nishizaki T, Ozaki S, et al.: Intratumoral cytogenetic heterogeneity detected by comparative genomic hybridization and laser scanning cytometry in human gliomas. Cancer Res 1998;58:4694-4700.
Huhn SL, Mohapatra G, Bollen A, et al.: Chromosomal abnormalities in glioblastoma multiforme by comparative genomic hybridization: Correlation with radiation treatment outcome. Clin Cancer Res 1999;5:1435-1443.
Uchida D, Kawamata H, Omotehara F, et al.: Over-expression of TSC-22 (TGF-beta stimulated clone-22) markedly enhances 5-fluorouracil-induced apoptosis in a human salivary gland cancer cell line. Lab Invest 2000;80:955-963.
Omotehara F, Uchida D, Hino S, et al.: In vivo enhancement of chemosensitivity of human salivary gland cancer cells by overexpression of TGF-beta stimulated clone-22. Oncol Rep 2000;7:737-740.
