Poster (Scientific congresses and symposiums)
MCF-7/BOS cells membrane proteome profiling for the analysis of plasmic proteins in pancreatic beta cells
Bertrand, Virginie; Massart, Anne-Cécile; Flamez, Daisy et al.
2007BioForum of ULg - 11th edition
 

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Keywords :
membrane protein; biotin; isolate; antibodies; diabetes; pancreatic islet; beta cell; PET; MCF-7/BOS; therapeutic targets
Abstract :
[en] Type I and type II diabetes are diseases characterized by the activation of the apoptotic processes in pancreatic islet of beta-cells. To highlight specific proteins of beta cells in medical imaging (positron emission tomography or PET), the development of antibodies directed against membrane markers of the beta cells is undertaken. These membrane markers have to be accessible to antibodies and should be potential therapeutic targets. In order to isolate membrane proteins of beta cells, preliminary tests were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain a pure membrane fraction to facilitate the analysis of the sample. To isolate transmembrane proteins, we compared two methods. The first one used a differential centrifugation to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. Our results shown that the labelling technique can provide 50% of membrane proteins. This second method will be used to isolate membrane proteins of pancreatic beta cells.
Research center :
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Endocrinology, metabolism & nutrition
Anatomy (cytology, histology, embryology...) & physiology
Biochemistry, biophysics & molecular biology
Chemistry
Author, co-author :
Bertrand, Virginie ;  Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Massart, Anne-Cécile ;  Université de Liège - ULiège > Chimie > Laboratoire de Spectrométrie de Masse L.S.M.
Flamez, Daisy;  Free University of Bruxelles (ULB) > Laboratory of Experimental Medicine
Eizirk, Decio L.;  Free University of Bruxelles (ULB) > Laboratory of Experimental Medicine
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Jackers, Pascale ;  Université de Liège - ULiège > Chimie > Laboratoire de Spectrométrie de Masse L.S.M.
Language :
English
Title :
MCF-7/BOS cells membrane proteome profiling for the analysis of plasmic proteins in pancreatic beta cells
Publication date :
11 October 2007
Event name :
BioForum of ULg - 11th edition
Event organizer :
ULg
Event place :
Liège, Belgium
Event date :
11/10/2007
Name of the research project :
‘Validation of tumoral biomarkers using proteomic methods’
Funders :
BioWin - BioWin : pôle santé de Wallonie [BE]
Funding text :
KeyMarker (Biowin Agreement) GIGA
Available on ORBi :
since 02 May 2017

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