Poster (Scientific congresses and symposiums)
MCF-7/BOS cells membrane proteome profiling for the analysis of plasmic proteins in pancreatic beta cells
Bertrand, Virginie; Massart, Anne-Cécile; Flamez, Daisy et al.
2008Belgian Society for Mass Spectrometry - BSMS2008
 

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Keywords :
diabetes; pancreatic islet; beta-cells; PET; membrane protein; MCF-7/BOS; membrane proteins; biotin; isolate; therapeutic targets; antibodies
Abstract :
[en] Type I and type II diabetes are diseases characterized by the activation of the apoptotic processes in pancreatic islet of beta-cells. To highlight specific proteins of beta cells in medical imaging (positron emission tomography or PET), the development of antibodies directed against membrane markers of the beta cells is undertaken. These membrane markers have to be accessible to antibodies and should be potential therapeutic targets. In order to isolate membrane proteins of beta cells, preliminary tests were conducted on MCF-7 / BOS cell line, immortal and easier to cultivate. The goal of this work is to obtain a pure membrane fraction to facilitate the analysis of the sample. To isolate transmembrane proteins, we compared two methods. The first one used a differential centrifugation to isolate membrane proteins. The second method labelled accessible extracellular domains at the surface of MCF-7 cells with biotin prior to differential centrifugation. The labelling technique used can provide 50% of membrane proteins. This second method will be used to isolate membrane proteins of pancreatic beta cells.
Research center :
CART - Centre Interfacultaire d'Analyse des Résidus en Traces - ULiège
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Biochemistry, biophysics & molecular biology
Endocrinology, metabolism & nutrition
Chemistry
Author, co-author :
Bertrand, Virginie ;  Université de Liège > Département de chimie (sciences) > Département de chimie (sciences)
Massart, Anne-Cécile ;  Université de Liège - ULiège > Chimie > Laboratoire de Spectrométrie de Masse L.S.M.
Flamez, Daisy;  Experimental Medecine Laboratory, Free University of Brussels, Brussels, Belgium
Eizirik, Decio L.;  Experimental Medecine Laboratory, Free University of Brussels, Brussels, Belgium
De Pauw, Edwin  ;  Université de Liège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Jackers, Pascale ;  Université de Liège - ULiège > Chimie > Laboratoire de Spectrométrie de Masse L.S.M.
Language :
English
Title :
MCF-7/BOS cells membrane proteome profiling for the analysis of plasmic proteins in pancreatic beta cells
Publication date :
15 February 2008
Event name :
Belgian Society for Mass Spectrometry - BSMS2008
Event organizer :
Janssen Pharmaceutica NV
Event place :
Beerse, Belgium
Event date :
15/02/2008
Audience :
International
Name of the research project :
'Validation of tumoral biomarkers using proteomic methods’
Funders :
BioWin - BioWin: Health Cluster of Wallonia [BE]
Funding text :
KeyMarker (Biowin Agreement)
Available on ORBi :
since 02 May 2017

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