Poster (Scientific congresses and symposiums)
A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Farhat, Khalil; Bodart, Gwennaëlle; RENARD, Jeanne de Chantal et al.
2017European Congress of Endocrinology
 

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Keywords :
GHRH; GH deficiency; Streptococcus pneumoniae
Abstract :
[en] In the framework of close interactions between the immune and neuroendocrine systems, Growth Hormone (GH) has been proposed to exert significant effects on the immune system, but there is not yet a consensus about GH immunomodulatory properties. These studies investigated the immune and anti-infectious response of dwarf Ghrh-/- mice presenting a severe deficiency of the GHRH/GH/IGF-1 axis. In basal conditions, thymic parameters and T-cell responses of Ghrh-/- mice were not severely affected but a constant B-cell lymphopaenia was observed. Thus, we investigated vaccine and anti-infectious responses of Ghrh-/- mice toward Streptococcus pneumonia, a B-dependent pathogen, Ghrh-/- mice were unable to trigger production of specific IgM and IgG against serotype 1 pneumococcal polysaccharide (PPS) after vaccination with either native PPS (Pnx23) or protein-PPS conjugate (Prev-13) vaccines. These vaccines both include the serotype 1 (our S.pneumoniae strain) and provide an effective protection in mice. A short GH supplementation to Ghrh-/- mice (1 daily injection of 1 mg/kg GH for 4 weeks) restored IgM and IgG response to Pnx23 vaccine but not to Prev-13. This suggests that GH could exert distinct impacts upon spenic areas. Furthermore, after intranasal instillation of a non-lethal dose (defined by the full clearance by WT C57BL/6 mice after 24h) of serotype 1 S.pneumoniae, Ghrh-/- mice exhibited a dramatic susceptibility. This was proved by a marked time-dependent increase in pulmonary bacterial, a septicemia already 24h after infection and a survival limit of 72h. We also observed a dramatic decrease in lung B- and T-cell populations and an increase in proportion of inflammatory macrophages. By contrast, wild-type and heterozygote mice completely cleared S.pneumoniae infection after 24h. In conclusion, our data show without ambiguity that the somatotrope GHRH/GH/IGF-1 axis plays an important and unsuspected role in defense against S.Pneumoniae.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Endocrinology, metabolism & nutrition
Immunology & infectious disease
Author, co-author :
Farhat, Khalil;  Université de Liège - ULiège > GIGA-I3 Centre d'Immunoendocrinologie
Bodart, Gwennaëlle ;  Centre Hospitalier Universitaire de Liège - CHU > Centre d'oncologie
RENARD, Jeanne de Chantal ;  Centre Hospitalier Universitaire de Liège - CHU > Service d'endocrinologie clinique
Desmet, Christophe  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
MOUTSCHEN, Michel  ;  Centre Hospitalier Universitaire de Liège - CHU > Service des maladies infectieuses - médecine interne
Beguin, Yves  ;  Université de Liège > GIGA-R : Hématologie
Baron, Frédéric  ;  Université de Liège > GIGA-R : Hématologie
Salvatori, Roberto;  Johns Hopkins University > Endocrinology
Geenen, Vincent ;  Université de Liège > Centre d'immunologie
Martens, Henri ;  Université de Liège > Centre d'immunologie
Language :
English
Title :
A surprising and dramatic neuroendocrine-immune phenotype of mice deficient in Growth Hormone-Releasing Hormone (GHRH)
Publication date :
23 May 2017
Number of pages :
Poster
Event name :
European Congress of Endocrinology
Event organizer :
European Society of Endocrinology
Event place :
Lisbonne, Portugal
Event date :
21-23 mai 2017
Audience :
International
Additional URL :
Name of the research project :
ARC SOMASTHYM
Funders :
Gouvernement du Liban + ARC
Available on ORBi :
since 23 April 2017

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