Article (Scientific journals)
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis.
Leclercq, Sophie; Derouaux, Adeline; Olatunji, Samir et al.
2017In Scientific Reports, 7, p. 43306
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Abstract :
[en] Bacteria utilize specialized multi-protein machineries to synthesize the essential peptidoglycan (PG) cell wall during growth and division. The divisome controls septal PG synthesis and separation of daughter cells. In E. coli, the lipid II transporter candidate FtsW is thought to work in concert with the PG synthases penicillin-binding proteins PBP3 and PBP1b. Yet, the exact molecular mechanisms of their function in complexes are largely unknown. We show that FtsW interacts with PBP1b and lipid II and that PBP1b, FtsW and PBP3 co-purify suggesting that they form a trimeric complex. We also show that the large loop between transmembrane helices 7 and 8 of FtsW is important for the interaction with PBP3. Moreover, we found that FtsW, but not the other flippase candidate MurJ, impairs lipid II polymerization and peptide cross-linking activities of PBP1b, and that PBP3 relieves these inhibitory effects. All together the results suggest that FtsW interacts with lipid II preventing its polymerization by PBP1b unless PBP3 is also present, indicating that PBP3 facilitates lipid II release and/or its transfer to PBP1b after transport across the cytoplasmic membrane. This tight regulatory mechanism is consistent with the cell's need to ensure appropriate use of the limited pool of lipid II.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Leclercq, Sophie ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Derouaux, Adeline ;  Université de Liège > GIGA-R : Virologie - Immunologie
Olatunji, Samir ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Fraipont, Claudine ;  Université de Liège > Centre d'ingénierie des protéines
Egan, Alexander J. F.
Vollmer, Waldemar
Breukink, Eefjan
Terrak, Mohammed  ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Language :
English
Title :
Interplay between Penicillin-binding proteins and SEDS proteins promotes bacterial cell wall synthesis.
Publication date :
24 February 2017
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
7
Pages :
43306
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 April 2017

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