Article (Scientific journals)
Differential expression and localization of glycosidic residues in in vitro- and in vivo-matured cumulus-oocyte complexes in equine and porcine species.
Accogli, Gianluca; Douet, Cecile; Ambruosi, Barbara et al.
2014In Molecular Reproduction and Development, 81 (12), p. 1115-35
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Keywords :
Animals; Cumulus Cells/metabolism; Female; Histocytochemistry; Horses/metabolism; In Vitro Techniques; Lectins; Oligosaccharides/metabolism; Oocytes/metabolism; Species Specificity; Statistics, Nonparametric; Swine/metabolism; Zona Pellucida/metabolism
Abstract :
[en] Glycoprotein oligosaccharides play major roles during reproduction, yet their function in gamete interactions is not fully elucidated. Identification and comparison of the glycan pattern in cumulus-oocyte complexes (COCs) from species with different efficiencies of in vitro spermatozoa penetration through the zona pellucida (ZP) could help clarify how oligosaccharides affect gamete interactions. We compared the expression and localization of 12 glycosidic residues in equine and porcine in vitro-matured (IVM) and preovulatory COCs by means of lectin histochemistry. The COCs glycan pattern differed between animals and COC source (IVM versus preovulatory). Among the 12 carbohydrate residues investigated, the IVM COCs from these two species shared: (a) sialo- and betaN-acetylgalactosamine (GalNAc)-terminating glycans in the ZP; (b) sialylated and fucosylated glycans in cumulus cells; and (c) GalNAc and N-acetylglucosamine (GlcNAc) glycans in the ooplasm. Differences in the preovulatory COCs of the two species included: (a) sialoglycans and GlcNAc terminating glycans in the equine ZP versus terminal GalNAc and internal GlcNAc in the porcine ZP; (b) terminal galactosides in equine cumulus cells versus terminal GlcNAc and fucose in porcine cohorts; and (c) fucose in the mare ooplasm versus lactosamine and internal GlcNAc in porcine oocyte cytoplasm. Furthermore, equine and porcine cumulus cells and oocytes contributed differently to the synthesis of ZP glycoproteins. These results could be attributed to the different in vitro fertilization efficiencies between these two divergent, large-animal models.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Accogli, Gianluca
Douet, Cecile
Ambruosi, Barbara
Martino, Nicola Antonio
Uranio, Manuel Filioli
Deleuze, Stefan  ;  Université de Liège - ULiège
Dell'Aquila, Maria Elena
Desantis, Salvatore
Goudet, Ghylene
Language :
English
Title :
Differential expression and localization of glycosidic residues in in vitro- and in vivo-matured cumulus-oocyte complexes in equine and porcine species.
Publication date :
2014
Journal title :
Molecular Reproduction and Development
ISSN :
1040-452X
eISSN :
1098-2795
Publisher :
John Wiley & Sons, Hoboken, United States - New York
Volume :
81
Issue :
12
Pages :
1115-35
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
(c) 2014 Wiley Periodicals, Inc.
Available on ORBi :
since 29 March 2017

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