[en] Age-related changes of antennal-active components of male labial gland extracts were studied in two closely related bumblebee species, Bombus terrestris and B. lucorum. In B. terrestris, compounds eliciting electroantennogram (EAG) responses of virgin queens were ethyl dodecanoate, 2,3-dihydrofarnesal, 2,3-dihydrofarnesol, hexadecan-1-ol, octadeca-9,12,15-trien-1-ol, and geranylcitronellol. Compounds that elicited EAG responses from queens of B. lucorum were ethyl dodecanoate, ethyl tetradec-7-enoate, ethyl tetradec-9-enoate, ethyl hexadec-9-enoate, hexadecan-1-ol, hexadec-7-enal, octadeca-9,12-dien-1-ol, octadeca-9,12,15-trien-1-ol, and octadecan-1-ol. Quantities of these compounds in the labial glands changed significantly over the lifetime of the respective males of the two species. In both species, concentrations of the respective compounds reached their maximum within seven days after eclosion. Subsequently, a rapid decrease in the amount of EAG-active compounds occurred in B. terrestris, whereas in B. lucorum the amount of active compounds stayed approximately constant or decreased at a slow rate. Microscopy showed that in B. terrestris secretory cells of the labial glands undergo apoptosis from the fifth to the tenth day of life, whilst in B. lucorum labial gland cells remain unchanged throughout the life of the males.
Disciplines :
Agriculture & agronomy Entomology & pest control Chemistry
Author, co-author :
Zacek, Petr
Kalinova, Blanka
Sobotnik, Jan
Hovorka, Oldrich
Ptacek, Vladimir
Coppee, Audrey
Verheggen, François ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Valterova, Irena
Language :
English
Title :
Comparison of age-dependent quantitative changes in the male labial gland secretion of Bombus terrestris and Bombus lucorum.
Publication date :
2009
Journal title :
Journal of Chemical Ecology
ISSN :
0098-0331
eISSN :
1573-1561
Publisher :
Kluwer Academic/Plenum Publishers, New York, United States
Ågren, L., Cederberg, G., and Svensson, G. 1979. Changes with age in ultrastructure and pheromone content of male labial glands in some bumblebee species (Hymenoptera, Apidae). Zoon 7:1-14.
Bergman, P., 1997. Chemical communication in bumblebee premating behaviour. Ph.D. Thesis, Göteborg University, Sweden.
Bergman, P., and Bergström, G. 1997. Scent marking, scent origin, specifity in male premating behavior of two Scandinavian bumblebees. J. Chem. Ecol. 23:1235-1251.
Bergström, G., Kullenberg, B., and Ställberg-Stenhagen, S. 1973. Studies on natural odoriferous compounds: VII. Recognition of two forms of Bombus lucorum L. (Hymenoptera, Apidae) by analysis of the volatile marking secretion from individual males. Chem. Scr. 4:174-182.
Bergström, G., Svensson, B.G., Appelgren M., and Groth I. 1981. Complexity of bumble bee marking pheromones: biochemical, ecological and systematical interpretations, pp. 175-183, in P. J. Howse and J.-L. Clément (eds.). Biosystematics of Social Insects. Academic Press, London, New York.
Bertsch, A., Schweer, H., Titze, A., and Tanaka, H. 2005. Male labial gland secretions and mitochondrial DNA markers support species status of Bombus cryptarum and B. magnus (Hymenoptera, Apidae). Insectes Soc. 52:45-54.
Calam, D. H. 1969. Species and sex-specific compounds from the heads of male bumblebees (Bombus spp.). Nature 221:856-857.
Coppée, A., Terzo, M., Valterová, I., and Rasmont, P. 2008. Intraspecific variation of the cephalic labial gland secretions in Bombus terrestris (L.) (Hymenoptera: Apidae). Chem. Biodiversity 5:2654-2661.
Kindl, J., Hovorka, O., Urbanová, K., and Valterová, I. 1999. Scent marking in male premating behavior of Bombus confusus. J. Chem. Ecol. 25:1489-1500.
Kullenberg, B., Bergström, G., and Ställberg-Stenhagen, S. 1970. Volatile components of the cephalic marking secretion of male bumblebees. Acta Chem. Scand. 24:1481-1483.
Kullenberg, B., Bergström, G., Bringer, B., Carlberg, B., and Cederberg, B. 1973. Observations on scent marking by Bombus and Psithyrus males and location of site of production of the secretion. Zoon Suppl. 1:25.
Lloyd, J. E. 1981. Sexual selection: Individuality, identification, and recognition in a bumblebee and other insects. Fla. Entomologist 64:89-107.
Morse, D. H. 1982. Behavior and ecology of bumble bees, pp. 245-322, in H. R. Hermann (ed.). Social Insects, vol. III. Academic Press, New York.
Paterson, H.E.H. 1993. Evolution and the Recognition Concept of Species. Collected writings. Johns Hopkins University Press, Baltimore.
Ptáček, V. 1999. Obtaining and Overwintering Young Bumble Bee (Hymenoptera Bombinae) Queens. - Insect Pollination in Greenhouses. APIMONDIA, ICPBR, Soesterberg, The Netherlands, 55-57.
Ptáček, V., Pernová, E., and Borovec, R. 2000. The two-queen cascade method as an alternative technique for starting bumble bee (Bombus, Hymenoptera, Apidae) colonies in laboratory (Preliminary study). Pszczel. Zesz. Nauk. 44:305-309.
Rasmont, P., Terzo, M., Aytekin, M., Hines, H., Urbanová, K., Cahlíková, L., and Valterová, I. 2005. Cephalic secretions of the bumblebee subgenus Sibiricobombus Vogt suggests Bombus niveatus Kriechbaumer and Bombus vorticosus Gerstaecker are conspecific (Hymenoptera, Apidae, Bombus). Apidologie 36:571-584.
Schremmer, F. 1972. Beobachtungen zum Paarungsverhalten der Männchen von Bombus confusus Schenk. Z. Tierpsychol. 31:503-512.
Šobotník, J., Kalinová, B., Cahlíková, L., Weyda, F., Ptáček, V., and Valterová, I. 2008: Age-dependent changes in structure and function of the male labial gland in Bombus terrestris. J. Insect Physiol. 54:204-214.
Svensson, B.G. 1980. Species-isolating mechanisms in male bumble bees (Hymenoptera, Apidae). Abstr. Upps. Diss. Fac. Sci. 549:1-42.
Svensson, B.G., and Bergström, G. 1977. Volatile marking secretions from the labial gland of North European Pyrobombus D. T. males (Hymenoptera, Apidae). Insectes Sociaux 24:213-224.
Tasei, J.-N., Moinard, C., Moreau, L., Himpens, B., and Guyonnaud, S. 1998. Relationship between aging, mating and sperm production in captive Bombus terrestris. J. Apicultural Res. 37:107-113.
Terzo, M., Valterová, I., Urbanová, K., and Rasmont, P., 2003. De la nécessité de redécrire les phéromones sexuelles des mâles de bourdons [Hymenoptera: Apidae, Bombini] publiées avant 1996 pour leur utilisation en analyse phylogénétique. Phytoprotection 83:39-49.
Terzo, M., Urbanova, K., Valterova, I., and Rasmont, P. 2005. Intra and interspecific variability of the cephalic labial glands' secretions in male bumblebees: the case of Bombus (Thoracobombus) ruderarius and B. (Thoracobombus) sylvarum [Hymenoptera, Apidae]. Apidologie 36:85-96.
Urbanová, K., Valterová, I., Hovorka, O. and Kindl, J. 2001. Chemotaxonomical characterisation of males of Bombus lucorum collected in the Czech Republic. Eur. J. Entomol. 98:111-115.
Valterová, I. and Urbanová, K. 1997. Chemical signals of bumblebees. Chem. Listy 91:846-857 (in Czech).
Valterová, I., Kunze, J., Gumbert, A., Luxová, A., Liblikas, I., Kalinová, B., and Borg-Karlson, A.-K. 2007. Male bumble bee pheromonal components in the scent of deceit pollinated orchids; unrecognized pollinator cues? Arthropod-Plant Interactions 1:137-145.
Villalobos, E.M., and Shelly, T.E. 1987. Observations on the behavior of male Bombus sonorus (Hymenoptera: Apidae). J. Kansas Ent. Soc. 60:541-548.
