[en] Beekeepers suspected maize. Zea mays L., treated with imidacloprid to result in substantial loss of honey bee (Hymenoptera: Apidae) colonies in Belgium. The objective of this study was to investigate the potential impact of maize grown from imidacloprid-treated seeds on honey bee mortality. A survey of 16 apiaries was carried out, and all maize fields treated or not with imidacloprid were located within a radius of 3,000 m around the observed apiaries. Samples of honey, beeswax, and bees were collected in three colonies per apiary and analyzed for pesticide contain by liquid chromatography-tandem mass spectrometry and gas chromatography-tandem mass spectrometry. We first found significant correlation between the number of colonies per apiary and the mortality rates in an apiary. In addition, this mortality rate was inversely correlated with the surface of maize fields treated and not with imidacloprid, suggesting that this pesticide do not interact with bees fitness. Moreover, a very large number of our samples contained acarcides either prohibited or ineffective against varroa destructor (Anderson & Trueman) (Acari: Varroidae), suggesting that the treatment method used by the beekeepers to be inadequate for mite control. Our results support the hypothesis that imidacloprid seed-treated maize has no negative impact on honey bees.
Disciplines :
Entomology & pest control
Author, co-author :
Nguyen, Bach Kim ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Epidémiologie et analyse des risques appl. aux sc. vétér.
Alix, A., and C. Vergnet. 2007. Risk assessment to honey bees: a scheme developed in France for non-sprayed systemic compounds. Pest Manag. Sci. 63: 1069-1080.
Bonmatin, J. M., I. Moineau, R. Charvet, C. Fleche, M. E. Colin, and E. R. Hengsch. 2003. A LC/APCI-MS/MS method for analysis of imidacloprid in soils, in plants, and in pollens. Anal. Chem. 75: 2027-2033.
Bonmatin, J. M.. F. A. Marchand, R. Charvet, I. Moineau, E. R. Bengsch, and M. E. Colin. 2005. Quantification of imidacloprid uptake in maize crops. J. Agric. Food Chem. 53: 5336-5341.
Chauzat, M. P., J. P. Faucon, A. C. Martel, J. Lachaize, N. Cougoule, and M. Aubert. 2006. A survey on pesticide residues in pollen loads collected by honey-bees (Apis meullifera) in France. J. Econ. Entomol. 99: 253-262.
Chauzat, M. P., and J. Pierre. 2005. L'importance du pollen pour l'abeille domestique: le pollen et ses composants. Bull. Tech. Apicole 32: 11-28.
Costanza, R, R. dArge, R. deGroot, S. Färber, M. Grasso, B. Hannon, K Limburg, S. Naeem, R. V. ONeill, J. Paruelo, et al. 1997. The value of the world's ecosystem services and natural capital. Nature (Lond.) 387: 253-260.
Cox-Foster, D. L., S. Conlan, E. C. Holmes, G. Palacios, J. D. Evans, N. A. Moran, P. L. Quan, T. Briese, M. Hornig, D. M. Geiser, et al. 2007. A metagenomic survey of microbes in honey bee colony collapse disorder. Science (Wash., D.C.) 318: 283-287.
Dagnelie, P. 1998. Statistique théorique et appliquée. Tome 2. Inférence statistique à une et à deux dimensions. De Boeck & Larcier, Paris, France.
Decourtye, A., E. Lacassie, and M. Pham-Delègue. 2003. Learning performance of honeybees (Apis mellifera L.) are differentially affected by imidacloprid according to season. Pest Manag. Sci. 59: 269-278.
Decourtye, A., J. Devillers, S. Cluzeau, M. Charreton, and M. Pham-Delègue. 2004. Effects of imidacloprid an delta-methrin on associative learning in honeybees under semifield and laboratory conditions. Ecotoxicol. Environ. Saf. 57: 410-419.
Doucet-Personemi, C., M. P. Halm, F. Touffet, A. Rortais, and G. Arnold. 2003. Imidacloprid utilisé en enrobage de semences (Gaucho) et troubles des abeilles-Rapport final. Comité scientifique et technique de l'étude multifactorielle des troubles des abeilles, Caen, France.
Faucon, J. P., L. Mathieu, M. Ribière, A.C. Martel, P. Dra-jnudel, S. Zeggane, C. Aurières, and M.F.A. Aubert. 2002. Honey bee winter mortality in France in 1999 and 2000. Bee World 83: 14-23.
Faucon, J. P., C. Aurières, P. Drajnudel, L. Mathieu, M. Ribière, A. C. Martel, S. Zeggane, M. P. Chauzat, and MFA. Aubert. 2005. Experimental study on the toxicity of imidacloprid given in syrup to honey bee (Apis mellifera) colonies. Pest Manag. Sci. 61: 111-125.
Free, J. B. 1993. Insect pollination of crops. Academic, London, United Kingdom.
Halm, M. P., A. Rortais, G. Arnold, J. N. Tasei, and S. Rault. 2006. New risk assessment approach for systemic insecticides: the case of honey bees and imidacloprid (Gaucho). Environ. Sci. Technol. 40: 2448-2454.
Haubruge, E., B. K. Nguyen, J. Widart, J.-P. Thome, P. Fick-ers, and E. Depauw. 2006. Le dépérissement de l'abeille domestique, Apis mellifera L., 1758 (Hymenoptera: Apidae): faits et causes probables. Notes Fauniques Gembloux 59: 3-21.
Higes, M., R. Martin, and A. Meana. 2006. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J. Invertebr. Pathol. 92: 93-95.
Johansen, C. A., and C. Brown. 1972. Toxicity of carbaryl contaminated pollen collected by honey bees. Environ. Entomol. 3: 385-396.
Keller, I.,P. Fluri, and A.Imdorf. 2005. Pollen nutrition and colony development in honey bees: part I. Bee World 86: 3-10.
Kevan, P. G. 1999. Pollinators as bioindicators of the state of the environment: species, activity and diversity. Agric. Ecosyst. Environ. 74: 373-393.
Klein, A. M., B. E. Vaissiere, J. H. Cane, I. Steffan-Dewenter, S. A. Cunningham, C. Kremen, and T. Tscharntke. 2007. Importance of pollinators in changing landscapes for world crops. Proc. R. Soc. B Biol. Sci. 274: 303-313.
Lefebvre, M., and Bruneau E. 2003. Suivi sanitaire d'urgence de ruchers présentant des symptomes de dépérissement. Rapport final. Projet FF 02/15 (414), Fonds budgétaire des Matières premières, Bruxelles, Belgium.
Lefebvre, M., and E. Bruneau. 2005. Etat des lieux du phénomène de dépérissement des ruchers en Région wallonne. Rapport final, convention DGNRE/CARL Namur, Belgium.
Maus, C., G. Cure, and R. Schmuck. 2003. Safety of imidacloprid seed dressing to honey bees: a comprehensive overview and compilation of the current state of knowledge. Bull. Insectol. 56: 51-57.
Michener, C. D. 2000. The bees of the world. John Hopkins University Press, Baltimore, MD.
Ministère-de-l'Agriculture-et-de-la-Pêche. 1999. Avis aux détenteurs et aux utilisateurs de semences de tournesol. J. Off. Repub. Fr. 38: 2413.
Ministère-de-1'Agriculture-et-de-la-Pêche. 2004. Avis aux détenteurs d'autorisation de mise sur le marché, aux distributeurs et aux utilisateurs du produit phytopharma-ceutique Gaucho. J. Off. Repub. Fr. 126: 79.
Naunen, R., U. Ebbinghaus-Kintscher, and R. Schmuck. 2001. Toxicity and nicotinic acetylcholine receptor interaction of imidacloprid and its metabolites in Apis mellifera (Hymenoptera: Apidae). Pest Manag. Sci. 57: 577-586.
Olroyd, B. P. 2007. What's killing American honey bees? PLoS 5(6): e168.
Otten, C. 2003. Daten und Fakten zu den Völkerverlusten. ADE 8: 6-8.
Pirard, C., J. Widart, B. K. Nguyen, C. Deleuze, L. Heudt, E. Haubruge, E. De Pauw and J.-F. Focant. 2007. Development and validation of a multi-residue method for pesticide determination in honey using on-column liquid-liquid extraction and liquid chromatography-tan-dem mass spectrometry. J. Chromatogr. A 1152: 116-123.
Rortais, A., G. Arnold, M. P. Halm, and F. Touffet-Briens. 2005. Modes of honeybees exposure to systemic insecticides: estimated amounts of contaminated pollen and nectar consumed by different categories of bees. Apidologie 36: 71-83.
Roulston, T. H., J. H. Cane, S. L. Buchmann. 2000. What governs protein content of pollen: pollinator preferences, pollen-pistil interactions, or phylogeny? Ecol. Monogr. 70: 617-643.
Schmidt, H. W. 1996. The reaction of bees under influence of the insecticid imidacoprid. Appendice 12, Proceedings of the 6th Internationa] Symposium on Hazards of Pesticides to Bees, 17-18 September, Braunschweig, Germany. D. Brasse, Braunschweig, Germany.
Schmidt-Hempel, P. 1998. Parasites in Socials insects. Princeton University Press, Princeton, NJ.
Schmuck, R., R. Schoning, A. Stork, and O. Schramel. 2001. Risk posed to honeybees (Apis mellifera L., Hymenoptera) by an imidacloprid seed dressing of sunflowers. Pest Manag. Sci. 57: 225-238.
Steffan-Dewenter, I., and A. Kuhn. 2003. Honeybee foraging in differentially structured landscapes. Proc. R. Soc. Lond. B 270: 569-575.
Suchail, S., D. Guez, and L. Belzunces. 2001. Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environ. Toxicol. Chem. 20: 2482-2486.
Vanengelsdorp, D., R. Underwood, D. Caron, and J. Hayes. 2007. An estimate of managed colony losses in the winter of 2006-2007: a report commissioned by the apiary inspectors of America. Am. Bee J. 147: 599-603.
Williams, I. H. 1996. Aspects of bee diversity and crop pollination in the European Union, pp. 63- 80. In H. Williams [ed.], The conservation of bees. Linnean Society of London and the International Bee Research Association, Academic, London, United Kingdom.
Winston, O. E. 1972. Social homeostasis and the superor-ganism. In The insect societies. The Belkup Press of Harvard University, Cambridge, MA.