DNA Labeling at Electron Microscopy

[en] Here, we describe a method for locating DNA on ultrathin sections. This technique is compatible with all usual fixation and embedding procedures and can be combined with cytochemical methods. Ultrathin sections are incubated in a medium containing terminal deoxynucleotidyl transferase (TdT) and various non-isotopic nucleotide analogs. The labeled nucleotides bound to the surface of ultrathin sections are then visualized by an indirect immunogold labeling technique. This high-resolution method provides a powerful tool for pinpointing the precise location of DNA within biological material, even where DNA is present in very low amounts.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Thelen, Nicolas ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Thiry, Marc ; Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire

Language :

English

Title :

DNA Labeling at Electron Microscopy

Publication date :

03 February 2017

Main work title :

Histochemistry of Single Molecules

Author, co-author :

Carlo, Pellicciari

Marco Biggiogera, Biggiogera

Publisher :

Springer, New York, United States

ISBN/EAN :

978-1-4939-6787-2

Collection name :

Volume 1560 of the series Methods in Molecular Biology,

Pages :

269-276

Peer reviewed :

Peer reviewed

Available on ORBi :

since 24 February 2017

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Bibliography

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