Article (Scientific journals)
Maturation of the Escherichia coli divisome occurs in two steps
Aarsman, Mirjam E. G.; Piette, André; Fraipont, Claudine et al.
2005In Molecular Microbiology, 55 (6), p. 1631-1645
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Abstract :
[en] Cell division proteins FtsZ ( FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed. To investigate whether this reflects a time dependent maturation of the divisome, the average cell age at which FtsZ, FtsQ, FtsW, PBP3 and FtsN arrive at their destination was determined by immuno- and GFP- fluorescence microscopy of steady state grown cells at a variety of growth rates. Consistently, a time delay of 14 - 21 min, depending on the growth rate, between Z-ring formation and the mid cell recruitment of proteins down stream of FtsK was found. We suggest a two-step model for bacterial division in which the Z-ring is involved in the switch from cylindrical to polar peptidoglycan synthesis, whereas the much later localizing cell division proteins are responsible for the modification of the envelope shape into that of two new poles.
Disciplines :
Microbiology
Biochemistry, biophysics & molecular biology
Author, co-author :
Aarsman, Mirjam E. G.;  Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology
Piette, André ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Fraipont, Claudine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Vinkenvleugel, Thessa M. F.;  Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
den Blaauwen, Tanneke;  Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology
Language :
English
Title :
Maturation of the Escherichia coli divisome occurs in two steps
Publication date :
March 2005
Journal title :
Molecular Microbiology
ISSN :
0950-382X
eISSN :
1365-2958
Publisher :
Blackwell Publishing Ltd, Oxford, United Kingdom
Volume :
55
Issue :
6
Pages :
1631-1645
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 December 2008

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