Reference : Maturation of the Escherichia coli divisome occurs in two steps
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/2074
Maturation of the Escherichia coli divisome occurs in two steps
English
Aarsman, Mirjam E. G. [Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology > > >]
Piette, André mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Fraipont, Claudine mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Vinkenvleugel, Thessa M. F. [Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology > > >]
Nguyen-Disteche, Martine [Université de Liège - ULg > > Centre d'ingénierie des protéines > >]
den Blaauwen, Tanneke [Swammerdam Institute for Life Sciences University of Amsterdam > Molecular cytology > > >]
Mar-2005
Molecular Microbiology
Blackwell Publishing Ltd
55
6
1631-1645
Yes (verified by ORBi)
International
0950-382X
Oxford
[en] Cell division proteins FtsZ ( FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed. To investigate whether this reflects a time dependent maturation of the divisome, the average cell age at which FtsZ, FtsQ, FtsW, PBP3 and FtsN arrive at their destination was determined by immuno- and GFP- fluorescence microscopy of steady state grown cells at a variety of growth rates. Consistently, a time delay of 14 - 21 min, depending on the growth rate, between Z-ring formation and the mid cell recruitment of proteins down stream of FtsK was found. We suggest a two-step model for bacterial division in which the Z-ring is involved in the switch from cylindrical to polar peptidoglycan synthesis, whereas the much later localizing cell division proteins are responsible for the modification of the envelope shape into that of two new poles.
http://hdl.handle.net/2268/2074

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