Reference : Influence of purified dietary fibre on bacterial protein synthesis in the large intestin...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Animal production & animal husbandry
http://hdl.handle.net/2268/20737
Influence of purified dietary fibre on bacterial protein synthesis in the large intestine of pigs, as measured by the gas production technique.
English
Bindelle, Jérôme mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Buldgen, André [Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > > Unité de Zootechnie > >]
Michaux, David [Faculté Universitaire des Sciences Agronomiques de Gembloux - FUSAGx > > Unité de Zootechnie > >]
Wavreille, José [Centre de Recherches Agronomiques - CRA W, Gembloux > Département de Productions et Nutrition Animales > > >]
Destain, Jean-Pierre mailto [Université de Liège - ULg > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Leterme, Pascal [Prairie Swine Centre, Saskaton, Canada > > > >]
2007
Livestock Science
Elsevier Science
109
232-235
Yes (verified by ORBi)
International
1871-1413
Amsterdam
The Netherlands
[en] Pig ; Carbohydrates ; Nitrogen excretion ; In vitro fermentation ; Fermentation in vitro
[fr] Porcin ; Hydrates de carbone ; Excrétion azotée
[en] Microbial fermentation of non-digestible carbohydrates in the pig's large intestine induces a shift of N excretion from urea in urine to bacterial protein in faeces. Experiments were carried out to measure the mineral N incorporation by the pig intestinal microflora using 5 purified carbohydrates in a gas-test: starch (S), cellulose (C), inulin (I), pectin (P) and xylan (X). Fermentation kinetics was modelled. N source in the buffer solution was replaced by 15N labelled NH4Cl. The bacterial N fixation was determined at mid-fermentation, measuring 15N incorporation into the solid phase of the buffer. The bacterial N fixation was higher (Pb0.001) with I and S (19.9 and 18.1 mg N/g incubated DM), compared to P, C and X (8.7, 5.9 and 5.5 respectively). Inulin and S were fermented also more rapidly, even if I (0.081 h−1) and C (0.074 h−1) showed lower half time fractional rate of degradation than S (0.153 h−1), P (0.133 h−1) and X (0.104 h−1). The insoluble dietary fibre content of the substrates was negatively correlated to bacterial N fixation (r=−0.957, P=0.011). The high crude protein content of P (32.5 mg g−1DM) might explain the lower impact of this substrate on bacterial N fixation, despite its rapid fermentation. Beside the proportion of insoluble fibre, the N content and the rate of fermentation seem to be the major factors influencing bacterial protein synthesis. Further studies including ingredients with variable content of indigestible protein and mean retention time in the pig's intestines are necessary.
Researchers
http://hdl.handle.net/2268/20737
10.1016/j.livsci.2007.01.126
http://www.elsevier.com/wps/find/journaldescription.cws_home/706547/description#description
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