Article (Scientific journals)
Expression of Bone Sialoprotein, a Bone Matrix Protein, in Human Breast Cancer
Bellahcene, Akeila; Merville, Marie-Paule; Castronovo, Vincenzo
1994In Cancer Research, 54 (11), p. 2823-6
Peer Reviewed verified by ORBi
 

Files


Full Text
BELLAHCENE A CANCER RES 1994.pdf
Publisher postprint (2.3 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
bone sialoprotein; breast cancer; microcalcifications
Abstract :
[en] Microcalcifications are often associated with human mammary lesions, particularly with breast carcinomas. To date, the molecular mechanism that leads to the deposition of hydroxyapatite in the mammary tissue has not been elucidated. Bone sialoprotein (BSP) is a glycoprotein the expression of which coincides with the appearance of the first hydroxyapatite crystals during bone development. In this study, we report the observation that BSP, a bone matrix protein, is expressed in human mammary cancer cells. Using an immunoperoxidase technique, we studied the expression of BSP in 79 breast lesions, including 28 benign and 51 malignant specimens. Two polyclonal antibodies, one directed against intact human BSP and the other against a synthetic peptide of BSP (residues 277-294), were used and gave identical results. Normal mammary glands expressed undetectable or barely detectable amounts of BSP, and the majority of the benign lesions examined were generally unstained (0) or weakly stained (1+). Most of the breast carcinoma specimens (around 87%) showed a significant increase (P = 0.0001) in BSP expression. Breast carcinomas with microcalcifications had the highest immunoreactivity (2+ or 3+) to BSP antibodies. This is the first demonstration that BSP expression is significantly increased in breast cancer. Expression of BSP by breast cancer cells could play a major role in the deposition of microcalcifications and in the preferred bone homing of breast cancer cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Oncology
Author, co-author :
Bellahcene, Akeila ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Labo de recherche sur les métastases
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire
Language :
English
Title :
Expression of Bone Sialoprotein, a Bone Matrix Protein, in Human Breast Cancer
Publication date :
01 June 1994
Journal title :
Cancer Research
ISSN :
0008-5472
eISSN :
1538-7445
Publisher :
American Association for Cancer Research, United States - Maryland
Volume :
54
Issue :
11
Pages :
2823-6
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Fondation contre le Cancer [BE]
European Community program BIOMED 1
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 31 August 2009

Statistics


Number of views
53 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
143
Scopus citations®
without self-citations
123

Bibliography


Similar publications



Contact ORBi