Article (Scientific journals)
Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.
Wang, Tiehui; Kono, Tomoya; Mira Monte, Milena et al.
2013In Molecular Immunology, 53 (4), p. 398-409
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Keywords :
Amino Acid Sequence; Animals; Conserved Sequence; Cytokines/pharmacology; Exons; Gene Expression Regulation/drug effects/immunology; Humans; Interleukins/genetics/immunology; Introns; Macrophage Colony-Stimulating Factor/genetics/immunology; Macrophages/cytology/immunology/metabolism; Molecular Sequence Data; Oncorhynchus mykiss/genetics/immunology; Phylogeny; Protein Isoforms/genetics/immunology; Receptor, Macrophage Colony-Stimulating Factor/genetics/immunology; Recombinant Proteins/genetics/immunology; Sequence Homology, Amino Acid
Abstract :
[en] The mononuclear phagocyte system is composed of monocytes, macrophages and dendritic cells and has crucial roles in inflammation, autoimmunity, infection, cancer, organ transplantation and in maintaining organismal homeostasis. Interleukin-34 (IL-34) and macrophage colony stimulating factor (MCSF), both signalling through the MCSF receptor, regulate the mononuclear phagocyte system. A single IL-34 and MCSF gene are present in tetrapods. Two types of MCSF exist in teleost fish which is resulted from teleost-wide whole genome duplication. In this report, we first identified and sequence analysed six IL-34 genes in five teleost fish, rainbow trout, fugu, Atlantic salmon, catfish and zebrafish. The fish IL-34 molecules had a higher identity within fish group but low identities to IL-34s from birds (27.2-33.8%) and mammals (22.2-31.4%). However, they grouped with tetrapod IL-34 molecules in phylogenetic tree analysis, had a similar 7 exon/6 intron gene organisation, and genes in the IL-34 loci were syntenically conserved. In addition, the regions of the four main helices, along with a critical N-glycosylation site were well conserved. Taken together these data suggest that the teleost IL-34 genes described in this report are orthologues of tetrapod IL-34. Comparative expression study of the three trout MCSFR ligands revealed that IL-34, MCSF1 and MCSF2 are differentially expressed in tissues and cell lines. The expression of MCSF1 and MCSF2 showed great variance in different tissues and cell lines, suggesting a role in the differentiation and maintenance of specific macrophage lineages in specific locations. The relatively high levels of IL-34 expression across different tissues suggests a homeostatic role of IL-34 for the macrophage lineage in fish. One striking observation in the present study was the lack of induction of MCSF1 and MCSF2 expression but the quick induction of IL-34 expression by PAMPs and inflammatory cytokines in cell lines and primary head kidney macrophages in rainbow trout. In a parasitic proliferative kidney disease (PKD) model, the expression of IL-34 but not the dominant MCSF2 was affected by PKD, suggesting an involvement of macrophage function in this disease model. Thus IL-34 expression is sensitive to inflammatory stimuli and may regulate macrophage biology once up-regulated.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Wang, Tiehui
Kono, Tomoya
Mira Monte, Milena ;  Université de Liège > Immunologie et vaccinologie
Kuse, Haruka
Costa, Maria M.
Korenaga, Hiroki
Maehr, Tanja
Husain, Mansourah
Sakai, Masahiro
Secombes, Christopher J.
Language :
English
Title :
Identification of IL-34 in teleost fish: differential expression of rainbow trout IL-34, MCSF1 and MCSF2, ligands of the MCSF receptor.
Publication date :
2013
Journal title :
Molecular Immunology
ISSN :
0161-5890
Publisher :
Elsevier, United Kingdom
Volume :
53
Issue :
4
Pages :
398-409
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2012 Elsevier Ltd. All rights reserved.
Available on ORBi :
since 16 January 2017

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