Article (Scientific journals)
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV).
Collet, Bertrand; Urquhart, Katy; Mira Monte, Milena et al.
2015In PLoS ONE, 10 (9), p. 0137767
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Keywords :
Animals; Biomarkers; Fish Diseases/immunology/virology; Hematocrit; Immunity; Isavirus/physiology; Orthomyxoviridae Infections/immunology/veterinary/virology; Salmo salar/immunology/virology
Abstract :
[en] Monitoring the immune response in fish over the progression of a disease is traditionally carried out by experimental infection whereby animals are killed at regular intervals and samples taken. We describe here a novel approach to infectiology for salmonid fish where blood samples are collected repeatedly in a small group of PIT-tagged animals. This approach contributes to the reduction of animals used in research and to improved data quality. Two groups of 12 PIT-tagged Atlantic salmon (Salmo salar) were i.p infected with Infectious Salmon Anaemia Virus (ISAV) or culture medium and placed in 1 m3 tanks. Blood samples were collected at 0, 4, 8, 12, 16, 21 and 25 days post infection. The viral load, immune and stress response were determined in individual fish by real-time quantitative PCR (QPCR) on the blood cells, as well as the haematocrit used as an indicator of haemolysis, a clinical consequence of ISAV infection. "In-tank" anaesthesia was used in order to reduce the stress related to chase and netting prior to sampling. The data were analysed using a statistical approach which is novel with respect to its use in fish immunology. The repeated blood collection procedure did not induce stress response as measured by HSP70 and HSP90 gene expression in the un-infected animals. A strong increase in viraemia as well as a significant induction of Mx and gammaIP gene expression were observed in the infected group. Interleukin 10 was found induced at the later stage of the infection whereas no induction of CD8 or gamma IFN could be detected. These results and the advantages of this approach are discussed.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Collet, Bertrand
Urquhart, Katy
Mira Monte, Milena ;  Université de Liège > Immunologie et vaccinologie
Collins, Catherine
Garcia Perez, Sandro
Secombes, Chris J.
Hall, Malcolm
Language :
English
Title :
Individual Monitoring of Immune Response in Atlantic Salmon Salmo salar following Experimental Infection with Infectious Salmon Anaemia Virus (ISAV).
Publication date :
2015
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States - California
Volume :
10
Issue :
9
Pages :
e0137767
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 January 2017

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