Euglenozoa; Trypanosomatidae; Dimeric mitochondrial complex V; F1FO ATP synthase; Electron microscopy; ATP synthasome
Abstract :
[en] Mitochondrial respiratory-chain complexes from Euglenozoa comprise classical subunits described in other eukaryotes (i.e. mammals and fungi) and subunits that are restricted to Euglenozoa (e.g. Euglena gracilis and Trypanosoma brucei). Here we studied the mitochondrial F1FO-ATP synthase (or Complex V) from the photosynthetic eukaryote E. gracilis in detail. The enzyme was purified by a two-step chromatographic procedure and its subunit composition was resolved by a three-dimensional gel electrophoresis (BN/SDS/SDS). Twenty-two different subunits were identified by mass-spectrometry analyses among which the canonical alpha, beta, gamma, delta, epsilon and OSCP subunits, and at least seven subunits previously found in Trypanosoma. The ADP/ATP carrier was also associated to the ATP synthase into a dimeric ATP synthasome. Single-particle analysis by transmission electron microscopy of the dimeric ATP synthase indicated that the structures of both the catalytic and central rotor parts are conserved while other structural features are original. These new features include a large membrane-spanning region joining the monomers, an external peripheral stalk and a structure that goes through the membrane and reaches the inter membrane space below the c-ring, the latter having not been reported for any mitochondrial F-ATPase.
Bouillenne, Fabrice ; Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Degand, Hervé
Morsomme, Pierre
González-Halphen, Diego
Remacle, Claire ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Cardol, Pierre ; Université de Liège > Département des sciences de la vie > Génétique et physiologie des microalgues
Language :
English
Title :
Atypical composition and structure of the mitochondrial dimeric ATP synthase from Euglena gracilis
Publication date :
12 January 2017
Journal title :
Biochimica et Biophysica Acta. Bioenergetics
ISSN :
0005-2728
eISSN :
1879-2650
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
1858
Issue :
4
Pages :
267-275
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
H2020 - 682580 - BEAL - Bioenergetics in microalgae : regulation modes of mitochondrial respiration, photosynthesis, and fermentative pathways, and their interactions in secondary algae
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] CE - Commission Européenne [BE]
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