Article (Scientific journals)
In-utero coxsackievirus B4 infection of the mouse thymus
Jaïdane, Hela; Halouani, Aymen; Jmii, H. et al.
2016In Clinical and Experimental Immunology, Sous presse
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Keywords :
Fetal thymus; Mouse model; Type B coxsackievirus; Vertical transmission; T-cell differentiation
Abstract :
[en] Type B coxsackievirus (CV-B) infections are involved frequently in the triggering of several autoimmune diseases such as myocarditis, dilated cardiomyopathy, pericarditis, pancreatitis, type 1 diabetes, encephalitis, thyroiditis or Sjo€gren’s syndrome. Serological and virological evidence suggests that maternal infections during pregnancy can play a role in the appearance of these diseases in offspring. The current study aims to explore the effect of an in-utero CV-B infection on the fetal thymus, the central site for programming immunological self-tolerance. In this perspective, female Swiss albino mice were inoculated intraperitoneally or orally with the diabetogenic CV-B4 E2 strain at gestational days 10 or 17. Offspring were killed at different post-inoculation times, and their thymuses were analysed for evidence of infection and alterations in thymic T cell subsets. In-utero CV-B infection of the thymus was demonstrated during the course of vertical transmission, as attested by viral RNA and infectious virus detection in most analysed samples. No histopathological changes were evident. Thymic T cells were not depleted, despite being positive for viral RNA. As evidenced by flow cytometry analysis, CV-B infection of the fetal thymus induced significant changes of thymic T cell populations, particularly with maternal inoculation at gestational day 10. Altogether, these findings suggest that CV-B infection of the fetal thymus may play an important role in the genesis of autoimmune diseases.
Research center :
GIGA-I3 - Giga-Infection, Immunity and Inflammation - ULiège
Disciplines :
Immunology & infectious disease
Author, co-author :
Jaïdane, Hela;  Université El Manar (Tunis) > Faculté des Sciences
Halouani, Aymen ;  Université El Manar (Tunis) > Faculté des Sciences
Jmii, H.;  Université El Manar (Tunis) > Faculté des Sciences
Elmastour, F.;  Université El Manar (Tunis) > Faculté des Sciences
Abdelkefi, S.;  Université El Manar (Tunis) > Faculté des Sciences
Bodart, Gwennaëlle ;  Université de Liège > Centre d'immunologie
Michaux, Hélène ;  Université de Liège > Centre d'immunologie
Chakroun, T.;  Université de Sousse (Tunisie) > Unité de recherche UR06SP05
Sane, F.;  Université de Lille 2 > Laboratoire de Virologie EA3610
Mokni, Moncef;  Université de Sousse (Tunisie) > Service d'Anatomopathologie
Geenen, Vincent ;  Université de Liège > Centre d'immunologie
Hober, Didier;  Université de Lille 2 > Laboratoire de Virologie EA3610
Aouni, Mahjoub;  Université de Monastir (Tunisie) > Laboratoire des Maladies Transmissibles
More authors (3 more) Less
Language :
English
Title :
In-utero coxsackievirus B4 infection of the mouse thymus
Publication date :
26 October 2016
Journal title :
Clinical and Experimental Immunology
ISSN :
0009-9104
eISSN :
1365-2249
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
Sous presse
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Ministère de l'enseignement supérieur et de la recherche scientifique de Tunisie
Available on ORBi :
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