In vitro evaluation of biocompatibility of uncoated thermally reduced graphene and carbon nanotube-loaded PVDF membranes with adult neural stem cell-derived neurons and glia
[en] Graphene, graphene-based nanomaterials (GBNs), and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies, the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here, we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly(vinylidene fluoride) (PVDF) membranes loaded with multi-walled CNTs (MWCNTs) using neural stem cells isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin- PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching, and on MWCNTs-loaded membranes oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks.
Research center :
Center for Education and Research on Macromolecules (CERM) CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Defteralı, Çağla; Instituto Cajal, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid, Spain > Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED-ISCIII), Madrid, Spain
Verdejo, Raquel; Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), Madrid, Spain
Majeed, Shahid; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
Boschetti-de-Fierro, Adriana; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
Méndez-Gómez, Héctor R.; Instituto Cajal, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid, Spain > Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED-ISCIII), Madrid, Spain
Díaz-Guerra, Eva; Instituto Cajal, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid, Spain > Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED-ISCIII), Madrid, Spain
Fierro, Daniel; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
Buhr, Kristian; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
Abetz, Clarissa; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
Martínez-Murillo, Ricardo; Instituto Cajal, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid, Spain
Vuluga, Daniela; University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Alexandre, Michaël; University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Thomassin, Jean-Michel ; University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Detrembleur, Christophe ; University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; University of Liège - ULiège > Complex and Entangled Systems from Atoms to Materials (CESAM), Center for Education and Research on Macromolecules (CERM)
Abetz, Volker; Helmholtz-Zentrum Geesthacht (HZG), Zentrum für Material- und Küstenforschung GmbH, Institut für Polymerforschung, Geesthacht, Germany
López-Manchado, Miguel Ángel; Instituto de Ciencia y Tecnología de Polímeros (ICTP-CSIC), Madrid, Spain
Vicario-Abejón, Carlos; Instituto Cajal, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid, Spain > Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED-ISCIII), Madrid, Spain
In vitro evaluation of biocompatibility of uncoated thermally reduced graphene and carbon nanotube-loaded PVDF membranes with adult neural stem cell-derived neurons and glia
Publication date :
06 December 2016
Journal title :
Frontiers in Bioengineering and Biotechnology
eISSN :
2296-4185
Publisher :
Frontiers Research Foundation, Lausanne, Switzerland
Volume :
4
Issue :
n° 64
Peer reviewed :
Peer Reviewed verified by ORBi
European Projects :
FP7 - 213277 - HARCANA - High Aspect Ratio Carbon-based Nanocomposites
Funders :
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] CE - Commission Européenne [BE]
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