Chemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time
[en] The chemical composition of essential oils obtained by hydrodistillation from aerial parts of Ocimum basilicum, Ocimum canum and Ocimum gratissimum from Benin as affected by harvesting time, were analyzed by GC-FID (Gas chromatography-Flame ionization detector) and GC-MS (Gas chromatography-Mass spectrometry). Based on the composition analysis, major components were as follows: estragol (43.0-44.7%) and linalool (24.6 29.8%) in O. basilicum oils; carvacrol (12.0-30.8%) and p-cymene (19.5-26.2%) in O. canum oils; thymol (28.3-37.7%) and γ-terpinene (12.5-19.3 %) in O. gratissimum oils. Disc diffusion and broth microdilution assays were used to evaluate the antibacterial activity of essential oils and their main components against two foodborne bacteria, Listeria monocytogenes and Salmonella enterica serotype Typhimurium. The tested oils and their components exhibited notable antimicrobial activities against L. monocytogenes and S. Typhimurium. The O. canum and O. gratissimum oils collected at 7h and 19h showed significant higher activities against L. monocytogenes and S. Typhimurium (MICs and MBCs 0.34 - 2.5 μL/mL) (p < 0.05), whereas O. basilicum showed lower activity (MICs and MBCs 2.0 - 8.0 μL/mL) at any daytime of harvest, the weakest being at 19h (MIC and MBC 12.0 - 32.0 μL/mL). The daytime of harvest can influence the composition of oils and their activities on bacteria.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Agriculture & agronomy Food science
Author, co-author :
Mith, Hasika; Institute of Technology of Cambodia, > Department of Chemical Engineering and Food Technology
Yayi-Ladékan, Eléonore; University of Abomey-Calavi > Laboratory of Pharmacognosy and Essential Oils
Dosso Sika Kpoviessi, Salomé; University of Abomey-Calavi > Faculty of Sciences and Techniques
Yaou Bokossa, Innocent; University of Abomey-Calavi > Faculty of Sciences and Techniques
Moudachirou, Mansourou; University of Abomey-Calavi > Laboratory of Pharmacognosy and Essential Oils
Daube, Georges ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Clinquart, Antoine ; Université de Liège > Département de sciences des denrées alimentaires (DDA) > Technologie des denrées alimentaires
Language :
English
Title :
Chemical composition and antimicrobial activity of essential oils of Ocimum basilicum, Ocimum canum and Ocimum gratissimum in function of harvesting time
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