[en] BACKGROUND: Interferons play an important role in innate immunity. Previous studies report deficiency in virus-induction of interferon (IFN)-alpha, -beta and -lambda in bronchial epithelial and bronchial lavage cells in atopic asthmatics. It is now recognized that asthma is a heterogeneous disease comprising different inflammatory phenotypes, some of which may involve innate immune activation in the absence of overt infection. OBJECTIVE: The aim of the study was investigate if the severity of asthma or a specific cellular sputum pattern may be linked to evidence of innate immune activation. METHODS: Here we investigate the expression of IFN-beta, IFN-lambda1 (IL-29), IFN-lambda2/3 (IL-28A/B) and the interferon-stimulated genes (ISGs) myxovirus resistance 1 (Mx1), oligoadenylate synthetase (OAS) and viperin in unstimulated sputum cells in 57 asthmatics (including 16 mild, 19 moderate and 22 severe asthma patients) and compared them with 19 healthy subjects. RESULTS: We observed increased expression of IFN-beta, IFN-lambda1/IL-29, OAS and viperin in asthmatic compared to healthy subjects while IL-28 was not expressed in any group. The overexpression was restricted to neutrophilic asthmatics (sputum neutrophils >/= 76%) while eosinophilic asthmatics (sputum eosinophils >/= 3%) did not differ from healthy subjects or even showed a lower expression of Mx1. No difference in interferon or ISG expression was seen according to clinical asthma severity. CONCLUSION AND CLINICAL RELEVANCE: Neutrophilic, but not eosinophilic, asthmatics display overexpression of IFN-beta, IFN-lambda1/IL-29 and ISGs in their sputum cells that may reflect ongoing innate immune activation. This article is protected by copyright. All rights reserved.
Disciplines :
Cardiovascular & respiratory systems
Author, co-author :
da Silva, Jane; Université de Liège
Hilzendeger, Clarissa ; Université de Liège > Département des sciences cliniques > Pneumologie - Allergologie
Moermans, Catherine ; Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie
SCHLEICH, FLorence ; Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
HENKET, Monique ; Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
Kebadze, Tatiana; National Heart and Lung Institute
Mallia, Patrick; National Heart and Lung Institute
Edwards, ichael John; National Heart and Lung Institute
Johnston, Sebastian Lennox; National Heart and Lung Institute
Louis, Renaud ; Université de Liège > Département des sciences cliniques > Pneumologie - Allergologie
Language :
English
Title :
Raised interferon beta, type 3 interferon and interferon stimulated genes - evidence of innate immune activation in neutrophilic asthma.
Publication date :
13 September 2016
Journal title :
Clinical and Experimental Allergy
ISSN :
0954-7894
eISSN :
1365-2222
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Peer reviewed :
Peer reviewed
Commentary :
This article is protected by copyright. All rights reserved.
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