[en] Calcium carbonate particles are promising candidates as proteins carriers for their controlled delivery in the body. The present paper aims at investigating the protein encapsulation by in situ precipitation of calcium carbonate particles prepared by a process based on supercritical CO2 and using a new type of degradable well-defined double hydrophilic block copolymers composed of poly(ethylene oxide) and polyphosphoester blocks acting as templating agent for the calcium carbonate. For this study, lysozyme was chosen as a model for therapeutic protein for its availability and ease of detection. It was found that by this green process, loading into the CaCO3 microparticles with a diameter about 2 mm can be obtained as determined by scanning electron microscopy. A protein loading up to 6.5% active lysozyme was measured by a specific bioassay (Micrococcus lysodeikticus). By encapsulating fluorescent-labelled lysozyme (lysozyme-FITC), the confocal microscopy images confirmed its encapsulation and suggested a core–shell distribution of lysozyme into CaCO3, leading to a release profile reaching a steady state at 59% of release after 90 min.
Research center :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Ergül, Zeynep ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Cordonnier, Thomas; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Debuigne, Antoine ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Clavignac, Brice; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Jérôme, Christine ; University of Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Boury, Frank; University of Angers France, INSERM U1066, Micro et Nanomedécines Biomimétiques, IBS
Language :
English
Title :
Protein encapsulation and release from PEO-b-polyphosphoester templated calcium carbonate particles
Publication date :
20 November 2016
Journal title :
International Journal of Pharmaceutics
ISSN :
0378-5173
eISSN :
1873-3476
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
513
Pages :
130-1
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique The European Community in the frame of the Erasmus Mundus Joint Doctorate Program Nanofar
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