Article (Scientific journals)
Cryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Tonus, Céline; Connan, Delphine; Waroux, Olivier et al.
2017In Theriogenology, 88, p. 197-206
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Keywords :
chicken primordial germ cells; cryopreservation; slow freezing; aseptic vitrification; genetic resources preservation; male and female germplasm; cellules germinales primordiales; poulet; cryopréservation; Congélation lente; vitrification aseptique; préservation des ressources génétiques
Abstract :
[en] In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared to controls, but the difference was significant only for vitrification. No difference was found between the two methods after flow cytometry analysis of SSEA-1 expression and RT-PCR on several factors related to PGCs phenotype. After one week in culture, all cryopreserved cells reached controls main morphological and expanding (viability/proliferation) features. However, slow freezing generated more unwanted cells clusters than vitrification. After injection of the PGCs into recipient embryos, vitrified PGCs showed a clear, yet not significant, tendency to colonize the gonad at a higher rate than slow frozen PGCs. Slow freezing in cryovials remains simple, inexpensive and less technically demanding than vitrification. Nevertheless, the intrinsic advantages of our aseptic vitrification method and the present study suggest that this should be considered as safer than classical slow freezing for cryopreserving chicken PGCs.
Research center :
FARAH - Fundamental and Applied Research for Animals and Health - ULiège
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Veterinary medicine & animal health
Biotechnology
Author, co-author :
Tonus, Céline  ;  Université de Liège > Département de morphologie et pathologie (DMP) > Embryologie
Connan, Delphine  ;  Université de Liège > Département de morphologie et pathologie (DMP) > Embryologie
Waroux, Olivier  ;  Université de Liège > Département de morphologie et pathologie (DMP) > Département de morphologie et pathologie (DMP)
Vandenhove, Benoit ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Embryologie
Wayet, Jérome ;  Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Embryologie
Gillet, Laurent  ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Desmecht, Daniel ;  Université de Liège > Département de morphologie et pathologie (DMP) > Pathologie spéciale et autopsies
Antoine, Nadine ;  Université de Liège > Département de morphologie et pathologie (DMP) > Histologie
Ectors, Fabien  ;  Université de Liège > GIGA-Technology platforms : Plate-forme transgénique
Grobet, Luc ;  Université de Liège > Département de morphologie et pathologie (DMP) > Embryologie
 These authors have contributed equally to this work.
Language :
English
Title :
Cryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Publication date :
15 January 2017
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier, New York, United States - New York
Volume :
88
Pages :
197-206
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Région wallonne : DGO6
Available on ORBi :
since 22 September 2016

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