Article (Scientific journals)
Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
Borgs, Laurence; Peyre, Elise; Alix, Philippe et al.
2016In Scientific Reports, 6, p. 33377
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Abstract :
[en] Some mutations of the LRRK2 gene underlie autosomal dominant form of Parkinson's disease (PD). The G2019S is a common mutation that accounts for about 2% of PD cases. To understand the pathophysiology of this mutation and its possible developmental implications, we developed an in vitro assay to model PD with human induced pluripotent stem cells (hiPSCs) reprogrammed from skin fibroblasts of PD patients suffering from the LRKK2 G2019S mutation. We differentiated the hiPSCs into neural stem cells (NSCs) and further into dopaminergic neurons. Here we show that NSCs bearing the mutation tend to differentiate less efficiently into dopaminergic neurons and that the latter exhibit significant branching defects as compared to their controls.
Research center :
Giga-Neurosciences - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Borgs, Laurence  ;  Université de Liège > Giga - Neurosciences
Peyre, Elise  ;  Université de Liège > GIGA - Neurosciences
Alix, Philippe ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
Hanon, Kevin ;  Université de Liège > GIGA - Neurosciences
Grobarczyk, Benjamin ;  Université de Liège > GIGA - Neurosciences
Godin, Juliette 
Purnelle, Audrey 
Krusy, Nathalie 
Maquet, Pierre  ;  Université de Liège > Département des sciences cliniques > Neurologie
Lefèbvre, Philippe ;  Université de Liège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie
Seutin, Vincent ;  Université de Liège > Département des sciences biomédicales et précliniques > Pharmacologie
Malgrange, Brigitte   ;  Université de Liège > GIGA - Neurosciences
Nguyen, Laurent   ;  Université de Liège > GIGA - Neurosciences
More authors (3 more) Less
 These authors have contributed equally to this work.
Language :
English
Title :
Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
Publication date :
2016
Journal title :
Scientific Reports
eISSN :
2045-2322
Publisher :
Nature Publishing Group, London, United Kingdom
Volume :
6
Pages :
33377
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 22 September 2016

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