Article (Scientific journals)
Growth and Freeze-Drying Optimization of Bifidobacterium crudilactis
Tanimomo, Jean; Delcenserie, Véronique; Taminiau, Bernard et al.
2016In Food and Nutrition Sciences, 7, p. 616-626
Peer reviewed
 

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Keywords :
Probiotic; Bifidobacteria; Crudilactis; Cryoprotectant; Freeze-Drying
Abstract :
[en] Bifidobacterium crudilactis FR62/b/3 belongs to a new population of bifidobacteria isolated from raw milk and raw milk cheese. The objective of this work was to study the large scale culture of the stain and its stability in a dry formulation. Growth rate of Bifidobacterium crudilactis FR62/b/3 was optimal at a pH of 5.0 and a temperature of 37˚C. At a temperature growth of 33˚C and a pH of 5.0, the stationary phase was reached after 22 h, the viable cell number and the mean dry biomass concentration were respectively of 8.3 × 109 CFU/mL and of 2.1 g/L. Resistance of Bifidobacterium crudilactis FR62/b/3 to freeze-drying and effect of a variety of cryoprotectants to maintain the viability were also evaluated. Sorbitol was the most suitable cryoprotectant for freeze-drying as well as storage whereas sucrose and monosodium glutamate were only efficient during storage.
Disciplines :
Microbiology
Author, co-author :
Tanimomo, Jean
Delcenserie, Véronique ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire
Taminiau, Bernard  ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Daube, Georges  ;  Université de Liège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Saint-Hubert, Catherine
Durieux, Alain
Language :
English
Title :
Growth and Freeze-Drying Optimization of Bifidobacterium crudilactis
Publication date :
2016
Journal title :
Food and Nutrition Sciences
ISSN :
2157-944X
eISSN :
2157-9458
Publisher :
Scientific Research, Irvine, United States - California
Volume :
7
Pages :
616-626
Peer reviewed :
Peer reviewed
Available on ORBi :
since 21 September 2016

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