Poster (Scientific congresses and symposiums)
Exploring potentialities of RanbP2-type Zinc Fingers in RNA-binding proteins design
De Franco, Simona; Vandenameele, Julie; Galleni, Moreno et al.
2016RNA 2016- The RNA Society of Japan 18th Annual Meeting and the 21st Annual Meeting of the RNA Society
 

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Keywords :
RNA-binding; zinc finger; specificity
Abstract :
[en] Transcriptomes consist of several classes of RNAs, whose roles are central to innumerable biological processes as well as diseases. These observations justify the increasing interest in the engineering of functionalized RNA-binding proteins (RBPs) to specifically manipulate RNA function. In this context, the RanBP2-type Zinc Finger (ZF) domain emerged as a suitable scaffold for single-stranded RBPs design. The present study aimed to identify the sequence-specificity of several naturally occurring RanBP2-type ZFs by in vitro selection and use the natural variation in their substrate to create arrays of ZF domains displaying customized specificity. A parallel kinetic and thermodynamic characterization of ssRNA-ZFs interaction was performed by Isothermal Titration Calorimetry (ITC) and BioLayer Interferometry (BLI), respectively. Although our data showed that only little variations in the recognized sequence could be observed for the studied ZFs, they contribute to elucidate the molecular basis for the RanBP2-type ZF-ssRNA interaction.
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
De Franco, Simona ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Vandenameele, Julie ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Galleni, Moreno ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Vandevenne, Marylène ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Language :
English
Title :
Exploring potentialities of RanbP2-type Zinc Fingers in RNA-binding proteins design
Publication date :
29 June 2016
Number of pages :
A0
Event name :
RNA 2016- The RNA Society of Japan 18th Annual Meeting and the 21st Annual Meeting of the RNA Society
Event place :
Kyoto, Japan
Event date :
du 28 juin 2016 au 2 juillet 2016
Audience :
International
Name of the research project :
Modular assembly of RanBP2-type Zinc figers to target single-stranded RNA
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE]
Available on ORBi :
since 19 September 2016

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