Article (Scientific journals)
Lung-resident eosinophils represent a distinct regulatory eosinophil subset
Mesnil, Claire; Raulier, Stéfanie; Paulissen, Geneviève et al.
2016In Journal of Clinical Investigation, 126 (9), p. 3275-3295
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Keywords :
eosinophils; lung; homeostasis
Abstract :
[en] Increases in eosinophil numbers are associated with infection and allergic diseases, including asthma, but there is also evidence that eosinophils contribute to homeostatic immune processes. In mice, the normal lung contains resident eosinophils (rEos), but their function has not been characterized. Here, we have reported that steady-state pulmonary rEos are IL-5–independent parenchymal Siglec-FintCD62L+CD101lo cells with a ring-shaped nucleus. During house dust mite–induced airway allergy, rEos features remained unchanged, and rEos were accompanied by recruited inflammatory eosinophils (iEos), which were defined as IL-5–dependent peribronchial Siglec-FhiCD62L–CD101hi cells with a segmented nucleus. Gene expression analyses revealed a more regulatory profile for rEos than for iEos, and correspondingly, mice lacking lung rEos showed an increase in Th2 cell responses to inhaled allergens. Such elevation of Th2 responses was linked to the ability of rEos, but not iEos, to inhibit the maturation, and therefore the pro-Th2 function, of allergen-loaded DCs. Finally, we determined that the parenchymal rEos found in nonasthmatic human lungs (Siglec-8+CD62L+IL-3Rlo cells) were phenotypically distinct from the iEos isolated from the sputa of eosinophilic asthmatic patients (Siglec-8+CD62LloIL-3Rhi cells), suggesting that our findings in mice are relevant to humans. In conclusion, our data define lung rEos as a distinct eosinophil subset with key homeostatic functions.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Mesnil, Claire  ;  Université de Liège > GIGA-Research
Raulier, Stéfanie  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Paulissen, Geneviève ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Xiao, Xue ;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Birrell, MA
Pirottin, Dimitri ;  Université de Liège > Département des sciences fonctionnelles (DSF) > Département des sciences fonctionnelles (DSF)
Janss, Thibaut ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Starkl, P
Ramery, Eve ;  Université de Liège > Dép. clinique des animaux de compagnie et des équidés (DCA) > Biochimie médicale
HENKET, Monique ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
SCHLEICH, FLorence ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
Radermecker, Coraline ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Thielemans, K
Thiry, Marc  ;  Université de Liège > Département des sciences de la vie > GIGA-R : Biologie cellulaire
Belvisi, MG;  Université de Liège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Louis, Renaud ;  Centre Hospitalier Universitaire de Liège - CHU > Service de pneumologie - allergologie
Desmet, Christophe  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Marichal, Thomas   ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
Bureau, Fabrice  ;  Université de Liège > Département des sciences fonctionnelles (DSF) > GIGA-R : Biochimie et biologie moléculaire
More authors (10 more) Less
 These authors have contributed equally to this work.
Language :
English
Title :
Lung-resident eosinophils represent a distinct regulatory eosinophil subset
Publication date :
2016
Journal title :
Journal of Clinical Investigation
ISSN :
0021-9738
eISSN :
1558-8238
Publisher :
American Society for Clinical Investigation, Ann Arbor, United States - Michigan
Volume :
126
Issue :
9
Pages :
3275-3295
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 13 September 2016

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