Reference : Modulation of intestinal urea cycle by dietary spermine in suckling rat
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/20133
Modulation of intestinal urea cycle by dietary spermine in suckling rat
English
Gharbi, Myriam [Université de Liège - ULg > Département des sciences de la motricité > Didactique des sciences de la santé >]
Powroznik, Brigitte [> > > >]
Mazzucchelli, Gabriel mailto [Université de Liège - ULg > > Centre interfac. d'analyse des résidus en traces (CART) >]
Deville, Christelle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Nollevaux, Géraldine [> > > >]
Rusu, Dorina [> > > >]
Dandrifosse, Guy mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Peulen, Olivier mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Service administratif de la Faculté (Médecine) >]
4-Nov-2005
Biochemical and Biophysical Research Communications
Academic Press Inc Elsevier Science
336
4
1119-1124
Yes (verified by ORBi)
International
0006-291X
San Diego
[en] argininosuccinate synthetase ; nutrient ; polyamine ; intestine ; urea cycle ; weaning
[en] Argininosuccinate synthetase, an ubiquitous enzyme in mammals, catalyses the formation of argininosuccinate, the precursor of arginine. Arginine is recognised as an essential amino acid in foetuses and neonates, but also as a conditionally essential amino acid in adults. Argininosuccinate synthetase is initially expressed in enterocytes during the developmental period, it disappeared from this organ then appeared in the kidneys. Although the importance of both intestinal and renal argininosuccinate synthetases has been recognised for a long time, nutrients have not yet been identified as inducers of the gene expression. In the context of a proteomic screening of intestinal modifications induced by dietary spermine in suckling rats, we showed that argininosuccinate synthetase and carbamoyl phosphate synthase disappeared from enterocytes after this treatment. The disappearance of argininosuccinate synthetase in small intestine was confirmed by immunodetection. Expression of carbamoyl phosphate synthase and argininosuccinate synthetase coding genes decreased also after spermine administration. Expression of other urea cycle enzyme coding genes was modulated by spermine administration: argininosuccinate lyase decreased and arginase increased. Our results fit with the developmental variation of argininosuccinate synthetase and carbamoyl phosphate synthase. Modulation of the gene expression for several urea cycle enzymes suggests a coordination between all the pathway steps and switch toward polyamine (or proline and glutamate) biosynthesis from ornithine. (c) 2005 Elsevier Inc. All rights reserved.
http://hdl.handle.net/2268/20133
10.1016/j.bbrc.2005.08.240

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
uree.pdfNo commentaryPublisher postprint248.54 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.