ORBi: Rentier-Delrue Françoise - Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli

Reference : Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/20108

Title :	Tilapia growth hormone: molecular cloning of cDNA and expression in Escherichia coli
Language :	English
Author, co-author :	Rentier-Delrue, Françoise [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique >]
	Swennen, D. [> > > >]
	Philippart, Jean-Claude [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Biologie du comportement - Ethologie et psychologie animale >]
	L'Hoir, C. [> > > >]
	Lion, M. [> > > >]
	Benrubi, O. [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Martial, Joseph [> > > >]
Publication date :	1989
Journal title :	DNA
Publisher :	Mary Ann Liebert, Inc.
Volume :	8
Issue/season :	4
Pages :	271-8
ISSN :	0198-0238
City :	New York
Country :	NY
Keywords :	[en] Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; Cloning, Molecular/methods ; DNA/biosynthesis/genetics ; Escherichia coli/genetics ; Fishes/genetics ; Growth Hormone/biosynthesis/genetics ; Molecular Sequence Data ; Plasmids ; RNA, Messenger/biosynthesis ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Species Specificity ; Trout/genetics
Abstract :	[en] A cDNA library was prepared from poly(A)+RNA extracted from tilapia Oreochromis niloticus anterior pituitaries. The recombinant clones carrying the cDNA sequence of tilapia growth hormone (tiGH) were selected using a fragment of the trout growth hormone (tGH) cDNA as hybridization probe. The nucleotide sequence of the full-length tiGH cDNA was determined. This cDNA encodes a protein of 204 amino acids, including the putative signal peptide of 17 amino acids. Mature tiGH cDNA was inserted in an Escherichia coli expression vector which led to the production of tiGH protein with a yield estimated to be 20% of the total bacterial proteins.
Permalink :	http://hdl.handle.net/2268/20108
	also: http://hdl.handle.net/2268/61319
Mentions required by the publisher for OA :	1989/05/01

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