Reference : Evidence of pre-prosomatostatin mRNA in human normal and tumoral anterior pituitary gland
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/20107
Evidence of pre-prosomatostatin mRNA in human normal and tumoral anterior pituitary gland
English
Pagesy, P. [> > > >]
Li, J. Y. [> >]
Rentier-Delrue, Françoise mailto [Université de Liège - ULg > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique >]
Le Bouc, Y. [> > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Peillon, F. [> > > >]
1989
Molecular Endocrinology
Endocrine Society
3
8
1289-94
Yes (verified by ORBi)
0888-8809
Chevy Chase
MD
[en] Adenoma/metabolism ; Adult ; Autoradiography ; Blotting, Northern ; Female ; Humans ; Hypothalamus/analysis ; Male ; Middle Aged ; Oligonucleotide Probes ; Pituitary Gland, Anterior/*analysis ; Pituitary Neoplasms/*analysis ; Poly A/analysis ; Protein Precursors/*genetics ; RNA, Messenger/analysis ; Somatostatin/*genetics
[en] Expression of the SRIH gene was investigated in six human normal anterior pituitaries, six GH-, three PRL-, three mixed GH/PRL-secreting and four nonsecreting adenomas. Total cellular RNA and poly(A+) mRNAs were analyzed by dot and Northern blot hybridization to a 3'-end labeled oligonucleotide probe specific for the human pre-proSRIH mRNA. A weak but detectable pre-proSRIH hybridization signal was present in human normal anterior pituitaries and in the four groups of adenomas. The size of this pre-proSRIH mRNA was indistinguishable from that found in our hypothalamic samples and close to that described in the literature. The wide variation of the signal intensity from one case to the other in each group of the different types of normal and tumoral antehypophyseal samples prevented establishment of any correlation between the level of pre-proSRIH mRNA and the nature of the pituitary tissue. The presence of SRIH mRNA in human normal and tumoral anterior pituitary tissues provides a sound basis to substantiate the hypothesis of a SRIH biosynthesis in the human anterior pituitary gland.
http://hdl.handle.net/2268/20107
http://mend.endojournals.org/cgi/content/abstract/3/8/1289
1989/08/01

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