|Reference : Triiodothyronine inhibits transcription from the human growth hormone promoter|
|Scientific journals : Article|
|Life sciences : Biochemistry, biophysics & molecular biology|
|Triiodothyronine inhibits transcription from the human growth hormone promoter|
|Morin, A. [> >]|
|Louette, J. [> > > >]|
|Voz, Marianne [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Tixier-Vidal, A. [> > > >]|
|Belayew, A. [> > > >]|
|Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]|
|Molecular & Cellular Endocrinology|
|North Holland Publishing Company|
|[en] Animals ; Gene Expression Regulation/*drug effects ; Genes/drug effects ; Growth Hormone/*genetics ; Herpes Simplex/genetics ; Pituitary Neoplasms/pathology ; Promoter Regions, Genetic/*drug effects ; Rats ; Recombinant Fusion Proteins/biosynthesis ; Thymidine Kinase/genetics ; Transcription, Genetic/*drug effects ; Triiodothyronine/*pharmacology ; Tumor Cells, Cultured/drug effects|
|[en] Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to the herpes virus thymidine kinase promoter (TK-hGH) were introduced into rat pituitary GC cells by DEAE-dextran transfection. Transient expression was followed as a function of triiodothyronine (T3) concentration. The hGH-CAT expression was specifically inhibited by T3 following a typical dose-response curve while hGH-GH gene expression was not significantly modified. The transient expression of TK-hGH increased as a function of T3 concentration. These results indicate that T3 exerts two opposite effects on hGH gene expression. First, it down-regulates expression by acting on the promoter; second, it up-regulates expression by acting on the structural part of the gene. These action could be due to regulation of transcription and mRNA stabilization, respectively.|
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