ORBi: Morin A. - Triiodothyronine inhibits transcription from the human growth hormone promoter

Reference : Triiodothyronine inhibits transcription from the human growth hormone promoter


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/20097

Title :	Triiodothyronine inhibits transcription from the human growth hormone promoter
Language :	English
Author, co-author :	Morin, A. [> >]
	Louette, J. [> > > >]
	Voz, Marianne [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
	Tixier-Vidal, A. [> > > >]
	Belayew, A. [> > > >]
	Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Publication date :	1990
Journal title :	Molecular & Cellular Endocrinology
Publisher :	North Holland Publishing Company
Volume :	71
Issue/season :	3
Pages :	261-7
Audience :	International
ISSN :	0303-7207
City :	Amsterdam
Country :	The Netherlands
Keywords :	[en] Animals ; Gene Expression Regulation/drug effects ; Genes/drug effects ; Growth Hormone/genetics ; Herpes Simplex/genetics ; Pituitary Neoplasms/pathology ; Promoter Regions, Genetic/drug effects ; Rats ; Recombinant Fusion Proteins/biosynthesis ; Thymidine Kinase/genetics ; Transcription, Genetic/drug effects ; Triiodothyronine/*pharmacology ; Tumor Cells, Cultured/drug effects
Abstract :	[en] Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to the herpes virus thymidine kinase promoter (TK-hGH) were introduced into rat pituitary GC cells by DEAE-dextran transfection. Transient expression was followed as a function of triiodothyronine (T3) concentration. The hGH-CAT expression was specifically inhibited by T3 following a typical dose-response curve while hGH-GH gene expression was not significantly modified. The transient expression of TK-hGH increased as a function of T3 concentration. These results indicate that T3 exerts two opposite effects on hGH gene expression. First, it down-regulates expression by acting on the promoter; second, it up-regulates expression by acting on the structural part of the gene. These action could be due to regulation of transcription and mRNA stabilization, respectively.
Permalink :	http://hdl.handle.net/2268/20097
Mentions required by the publisher for OA :	1990/07/09

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