Reference : Multihormonal regulation of the human prolactin gene expression from 5000 bp of its upst...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/20089
Multihormonal regulation of the human prolactin gene expression from 5000 bp of its upstream sequence
English
Berwaer, M. [> > > >]
Monget, P. [> > > >]
Peers, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
Mathy, Marianne [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Bellefroid, Eric J. [> > > >]
Davis, J. R. [> > > >]
Belayew, A. [> > > >]
Martial, Joseph mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >]
1991
Molecular & Cellular Endocrinology
North Holland Publishing Company
80
1-3
53-64
Yes (verified by ORBi)
0303-7207
Amsterdam
The Netherlands
[en] Animals ; Base Sequence ; Cell Line ; Chromosome Walking ; Cloning, Molecular ; Exons ; *Gene Expression Regulation ; Humans ; Molecular Sequence Data ; Prolactin/*genetics ; Rats ; *Regulatory Sequences, Nucleic Acid ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Transcription, Genetic
[en] We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown to provide tissue-specific transient expression in rat pituitary GH3 cells. Multihormonal response was found in this transient expression assay, leading to significant 2- to 5-fold induction by addition of 8-chlorophenylthio-cyclic AMP, thyrotropin-releasing hormone, epidermal growth factor, basic fibroblast growth factor, phorbol myristate acetate, a calcium channel agonist (Bay K-8644) and triiodothyronine. A 3-fold inhibition was observed in the presence of the glucocorticoid agonist dexamethasone. The sequence of the hPRL promoter was determined up to coordinate -3470. Computer similarity search between the rat and human sequences showed two highly conserved regions corresponding to the proximal and distal tissue specific enhancers described in both PRL promoters.
http://hdl.handle.net/2268/20089
1991/09/01

There is no file associated with this reference.

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.