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| Reference : Multihormonal regulation of the human prolactin gene expression from 5000 bp of its upst... |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology | |||
| http://hdl.handle.net/2268/20089 | |||
| Multihormonal regulation of the human prolactin gene expression from 5000 bp of its upstream sequence | |
| English | |
| Berwaer, M. [> > > >] | |
| Monget, P. [> > > >] | |
Peers, Bernard  [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] | |
| Mathy, Marianne [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >] | |
| Bellefroid, E. [> > > >] | |
| Davis, J. R. [> > > >] | |
| Belayew, A. [> > > >] | |
| Martial, Joseph [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire >] | |
| 1991 | |
| Molecular & Cellular Endocrinology | |
| North Holland Publishing Company | |
| 80 | |
| 1-3 | |
| 53-64 | |
| 0303-7207 | |
| Amsterdam | |
| The Netherlands | |
| [en] Animals ; Base Sequence ; Cell Line ; Chromosome Walking ; Cloning, Molecular ; Exons ; *Gene Expression Regulation ; Humans ; Molecular Sequence Data ; Prolactin/*genetics ; Rats ; *Regulatory Sequences, Nucleic Acid ; Restriction Mapping ; Sequence Homology, Nucleic Acid ; Transcription, Genetic | |
| [en] We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown to provide tissue-specific transient expression in rat pituitary GH3 cells. Multihormonal response was found in this transient expression assay, leading to significant 2- to 5-fold induction by addition of 8-chlorophenylthio-cyclic AMP, thyrotropin-releasing hormone, epidermal growth factor, basic fibroblast growth factor, phorbol myristate acetate, a calcium channel agonist (Bay K-8644) and triiodothyronine. A 3-fold inhibition was observed in the presence of the glucocorticoid agonist dexamethasone. The sequence of the hPRL promoter was determined up to coordinate -3470. Computer similarity search between the rat and human sequences showed two highly conserved regions corresponding to the proximal and distal tissue specific enhancers described in both PRL promoters. | |
| http://hdl.handle.net/2268/20089 | |
| 1991/09/01 |
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