Article (Scientific journals)
Monitoring of anatabine release by methyl jasmonate elicited BY-2 cells using surface-enhanced Raman scattering
De Bleye, Charlotte; Dumont, Elodie; Dispas, Amandine et al.
2016In Talanta, 160, p. 754-760
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Keywords :
Surface-enhanced Raman scattering; Monitoring of anatabine release; BY-2 cells; Calibration in the matrix; Gold nanoparticles
Abstract :
[en] A new application of surface-enhanced Raman scattering (SERS) in the field of plant material analysis is proposed in this study. The aim was to monitor the release of anatabine by methyl jasmonate (MeJa) elicited Bright Yellow-2 (BY-2) cells. Gold nanoparticles (AuNps) were used as SERS substrate. The first step was to study the SERS activity of anatabine in a complex matrix comprising the culture medium and BY-2 cells. The second step was the calibration. This one was successfully performed directly in the culture medium in order to take into account the matrix effect, by spiking the medium with different concentrations of anatabine, leading to solutions ranging from 250 to 5000 µg L-1. A univariate analysis was performed, the intensity of a band situated at 1028 cm-1, related to anatabine, was plotted against the anatabine concentration. A linear relationship was observed with a R2 of 0.9951. During the monitoring study, after the MeJa elicitation, samples were collected from the culture medium containing BY-2 cells at 0, 24h, 48h, 72h and 96h and were analyzed using SERS. Finally, the amount of anatabine released in the culture medium was determined using the response function, reaching a plateau after 72h of 82 µg of anatabine released / g of fresh weight (FW) MeJa elicited BY-2 cells.
Research center :
Centre Interfacultaire de Recherche du Médicament - CIRM
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
De Bleye, Charlotte  ;  Université de Liège > Département de pharmacie > Chimie analytique
Dumont, Elodie ;  Université de Liège > Département de pharmacie > Chimie analytique
Dispas, Amandine  ;  Université de Liège > Département de pharmacie > Chimie analytique
Hubert, Cédric  ;  Université de Liège > Département de pharmacie > Chimie analytique
Sacre, Pierre-Yves  ;  Université de Liège > Département de pharmacie > Chimie analytique
Netchacovitch, Lauranne ;  Université de Liège > Département de pharmacie > Chimie analytique
De Muyt, Bénédicte;  Université de Liège - ULiège > Département des Sciences de la Vie > Biologie Moléculaire et Biotechnologie Végétales
Kevers, Claire ;  Université de Liège > Département des sciences de la vie > Département des sciences de la vie
Dommes, Jacques ;  Université de Liège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Hubert, Philippe  ;  Université de Liège > Département de pharmacie > Chimie analytique
Ziemons, Eric  ;  Université de Liège > Département de pharmacie > Département de pharmacie
Language :
English
Title :
Monitoring of anatabine release by methyl jasmonate elicited BY-2 cells using surface-enhanced Raman scattering
Publication date :
August 2016
Journal title :
Talanta
ISSN :
0039-9140
eISSN :
1873-3573
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
160
Pages :
754-760
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Available on ORBi :
since 08 August 2016

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