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The use of protein-polysaccharide complexes to enhance protein delivery in CaCO3 microparticles
Ramalapa, Bathabile; Crasson, Oscar; Vandevenne, Marylène et al.
2016FADEX 2016 Nanoparticles for Medicine
 

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Keywords :
calcium carbonate; protein delivery; protein-polysaccharide complexes
Abstract :
[en] The role of protein therapeutics in modern medicine has increased considerably due to their potential to perform complex functions in the body that cannot be mimicked by simple chemical compounds. To increase the safety, stability and efficacy of protein therapeutics; there exists a need for delivery systems that are biocompatible, prevent premature degradation of bioactives and allow targeted delivery and controlled release. Calcium carbonate (CaCO3) has gained great favour for employment in protein delivery due to possibilities of controlling size, morphology and crystalline forms of particles by tuning the synthesis conditions. The aim of the present work was to assess the significance of polysaccharide-protein complexes in enhancing the encapsulation of proteins in CaCO3 microspheres. A Chitin Binding Domain (ChBD), reported to have affinity for hyaluronic acid, was inserted on β-lactamase enzyme to develop a chimeric protein. The chimeric protein retained the activity of the enzyme and the binding properties and was encapsulated in CaCO3 microspheres by a super critical CO2 (ScCO2) process using hyaluronic acid as a templating agent. The particles were characterised in terms of size, zeta potential, morphology and protein loading. The results obtained confirmed the affinity of the ChBD to hyaluronic acid towards the production of stable, vaterite microparticles. Protein assays demonstrated that the ChBD enhanced the encapsulation of protein by up to 10 fold. Confocal images also suggested high encapsulation of the chimeric protein compared to native protein. Thus the production of polysaccharide-protein complexes seems effective in enhancing the encapsulation of proteins in CaCO3 microparticles using the ScCO2 process.
Research center :
Centre d'Ingénierie des protéines - Université de Liège
INSERM U1066, Micro et Nanomédecines Biomimétiques - Université d'Angers
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ramalapa, Bathabile ;  Université de Liège - ULiège > Form. doct. sc. (chimie - paysage)
Crasson, Oscar ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Vandevenne, Marylène ;  Université de Liège > Département des sciences de la vie > Centre d'ingénierie des protéines
Galleni, Moreno ;  Université de Liège > Département des sciences de la vie > Macromolécules biologiques
Boury, Frank;  Université d'Angers > INSERM U1066, Micro et Nanomédecines Biomimétiques
Cordonnier, Thomas;  Université d'Angers > INSERM U1066, Micro et Nanomédecines Biomimétiques
Language :
English
Title :
The use of protein-polysaccharide complexes to enhance protein delivery in CaCO3 microparticles
Publication date :
22 March 2016
Event name :
FADEX 2016 Nanoparticles for Medicine
Event organizer :
French-American Doctoral Exchange
Event place :
Grenoble, France
Event date :
du 21 mars au 25 mars
Audience :
International
Funders :
Erasmus Mundus Nanofar Program
Available on ORBi :
since 02 August 2016

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