[en] This study evaluates the trophic effects of three neurotrophins on traumatized adult auditory neurons in culture, and the presence of these neurotrophins in cochlear nucleus tissue. BDNF and NT-3 promoted survival but very limited neuritogenesis by adult auditory neurons in vitro, while NGF, although without a survival effect, evoked a robust neuritic outgrowth response when combined with BDNF. Messenger RNAs that encode for NGF, BDNF and NT-3 were detected by RT-PCR in RNA extracts from adult cochlear nuclei tissue. Based on these in vitro and in vivo findings, we propose NT-3 as the agent of the peripheral target-derived survival promoting effect and NGF, BDNF, and NT-3 as mediators of trophic influences originating from the central target (i.e. cochlear nucleus).
Disciplines :
Otolaryngology
Author, co-author :
Lefèbvre, Philippe ; Université de Liège > Département des sciences cliniques > Oto-rhino-laryngologie et audiophonologie
Spoendlin H. Arch Klin Exp Ohr 200, 275-291 (1972).
Koitchev K, Aran JM, Iranov E et al. Acta Otolaryngol 102, 31-39 (1985).
Lefebvre PP, Weber T, Rigo J-M et al. Hear Res 58, 185-192 (1992).
Lefebvre PP, Leprince P, Weber T et al. Brain Res 507, 254-280 (1990).
Van de Water TR, Lefebvre PP, Liu W et al. Abstracts of XVIth Meeting ARO, St Petersburg 47, 135 (1992).
Avila MA, Varela-Nieto I and Romero G. Dev Biol 159, 266-275 (1993).
Davies AM and Lindsay RM. Dev Biol 111, 62-72 (1985).
Lindsay RM, Barde Y-A, Davies AM et al. J Cell Sci Suppl. 3, 115-129 (1985).
Ylikoski J, Pirvola U, Moshnyakov M et al. Hear Res 65, 69-78 (1992).
Bottenstein JE and Sato G. Proc Natl Acad Sci USA 76, 514-517 (1979).
Innis MA, Gelfand DH, Sninsky JJ et al. PCR Protocols. A guide to methods and application. New York: Academic Press, 1990: 1-329.
Ausubel FM, Brent R, Kingston RE et al. The polymerase chain reaction. In: Ausubel FM, Brent R, Kinston RE, Moore DD, Seidman JG, Smith JA, Struhl K, eds. Current protocols in molecular biology Volume 2. New York: Wiley Intersciences, 1987: 15.1-15.4.
Klein R, Jing S and Nanduri V. Cell 65, 189-197 (1991).
Klein R, Nanduri V, Jing S et al. Cell 56, 395-403 (1991).
Lamballe F, Klein R and Barbacid M. Cell 66, 967-979 (1991).
Davies AM, Lee KF and Jaenisch R. Neuron 11/4, 565-574 (1993).
Galinovic-Schwartz V, Lefebvre PP, Liu W et al. Abstracts of XVIth Meeting ARO St Petersburg 144, 435 (1992).
Lefebvre PP, Malgrange B, Staecker H et al. Science 260/5106, 692-694 (1993).
Ryals BM, Ten Eyck B and Westbrook EW. Hear Res 43, 81-90 (1989).
