Article (Scientific journals)
Capillary electrophoresis method to determine siRNA complexation with cationic liposomes
Furst, Tania; Bettonville, Virginie; Farcas, Elena et al.
2016In Electrophoresis
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Keywords :
lisomes; siRNA; capillary electrophoresis
Abstract :
[en] Small interfering RNA (siRNA) inducing gene silencing has great potential to treat many human diseases. To ensure effective siRNA delivery, it must be complexed with an appropriate vector, generally nanoparticles. The nanoparticulate complex requires an optimal physiochemical characterization and the complexation efficiency has to be precisely determined. The methods usually used to measure complexation are gel electrophoresis and RiboGreen® fluorescence-based assay. However, those approaches are not automated and present some drawbacks such as the low throughput and the use of carcinogenic reagents. The aim of this work is to develop a new simple and fast method to accurately quantify the complexation efficiency. In this research, capillary electrophoresis (CE) was used to determine the siRNA complexation with cationic liposomes. The short-end injection mode applied enabled siRNA detection in less than 5 min. Moreover, the CE technique offers many advantages compared to the other classical methods. It is automated, does not require sample preparation and expensive reagents. Moreover, no mutagenic risk is associated to CE approach since no carcinogenic product is used. Finally, this methodology can also be extended to the characterization of other types of nanoparticles encapsulating siRNA, such as cationic polymeric nanoparticles.
Research center :
CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège
Disciplines :
Biotechnology
Author, co-author :
Furst, Tania ;  Université de Liège > Département de pharmacie > Pharmacie galénique
Bettonville, Virginie ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Farcas, Elena ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Frère, Antoine ;  Université de Liège > Département de pharmacie > Pharmacie galénique
Lechanteur, Anna  ;  Université de Liège > Département de pharmacie > Pharmacie galénique
Evrard, Brigitte  ;  Université de Liège > Département de pharmacie > Pharmacie galénique
Fillet, Marianne ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Piel, Géraldine ;  Université de Liège > Département de pharmacie > Pharmacie galénique
Servais, Anne-Catherine  ;  Université de Liège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Capillary electrophoresis method to determine siRNA complexation with cationic liposomes
Publication date :
July 2016
Journal title :
Electrophoresis
ISSN :
0173-0835
eISSN :
1522-2683
Publisher :
Vch Publishers, Weinheim, Germany
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 06 July 2016

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