ORBi: Wilmotte Annick - Molecular and pigment studies of the picophytoplankton in a region of the Southern Ocean (42-54 degrees S, 141-144 degrees E) in March 1998

Reference : Molecular and pigment studies of the picophytoplankton in a region of the Southern Ocean...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Environmental sciences & ecology Life sciences : Aquatic sciences & oceanology Physical, chemical, mathematical & earth Sciences : Earth sciences & physical geography
	To cite this reference:	http://hdl.handle.net/2268/19900

Title :	Molecular and pigment studies of the picophytoplankton in a region of the Southern Ocean (42-54 degrees S, 141-144 degrees E) in March 1998
Language :	English
Author, co-author :	Wilmotte, Annick [Université de Liège - ULg > Département des sciences de la vie > Enzymologie >]
	Demonceau, Caroline [> >]
	Goffart, Anne [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Océanologie >]
	Hecq, Jean-Henri [Université de Liège - ULg > Département des sciences et gestion de l'environnement > Océanologie >]
	Demoulin, Vincent [Université de Liège - ULg > Département des sciences de la vie > Algologie, mycologie et systématique expérimentale >]
	Crossley, A. C. [> > > >]
Publication date :	2002
Journal title :	Deep-Sea Research Part II, Topical Studies in Oceanography
Publisher :	Pergamon-Elsevier Science Ltd
Volume :	49
Issue/season :	16
Pages :	3351-3363
Audience :	International
ISSN :	0967-0645
City :	Oxford
Keywords :	[en] picocyanobacteria ; ribosomal RNA ; Southern Ocean ; diversity ; temperature
Abstract :	[en] Seven filtered seawater samples (depths between 30 and 55 m) collected during the SAZ project of the Austral summer of 1997-1998 were used for a simultaneous study of the picophytoplankton pigments based on high-performance liquid chromatography (HPLC) analyses and flow cytometry, and of the molecular diversity of the picophytoplankton based on their rDNA sequences. The sampling sites could be divided into three temperature zones, distinguished by their proximity to the Sub-Antarctic and Polar Fronts. HPLC analysis of total chlorophylls and carotenoids showed fairly low phytoplankton concentrations (77-262 ng chl al(-1)), with minimal values of the pigments in the two samples of the Polar Front Zone around 54degreesS (water temperature of 4degreesC at time of collection). In this zone, a similar decrease of particles, identified as cyanobacteria on the basis of their fluorescence, was observed by flow cytometry. Sequences very similar to the 16S rDNA sequence of Synechococcus WH8103 were present in all samples. This Synechococcus genotype is thus found in the Southern Ocean in addition to the Atlantic and Pacific locations where it has been previously observed. The yield of PCR products was lower in the two samples taken in the Polar Front Zone, showing a good agreement between molecular and pigment data. 16S rDNA sequences of plastids of eukaryotic algae also were retrieved, mostly related to those of an environmental clone called OM164, which has not been cultivated but has phylogenetic affinities to the Raphidophyceae. (C) 2002 Elsevier Science Ltd. All rights reserved.
Funders :	BELSPO ‘‘Recherches scientifiques sur l’Antarctique-phase IV’’
Name of the research project :	A4/DD/B21-23
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/19900

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