No full text
Article (Scientific journals)
Binding of a 100-kDa ubiquitous factor to the human prolactin promoter is required for its basal and hormone-regulated activity
Peers, Bernard; Nalda, Asunción M; Monget, Philippe et al.
1992In European Journal of Biochemistry, 210 (1), p. 53-8
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Keywords :
Base Sequence; Blotting, Southern; Blotting, Western; Cells, Cultured; Cyclic AMP/metabolism; Humans; Molecular Sequence Data; Mutation; Oligonucleotides; Plasmids; Prolactin/*genetics/metabolism; *Promoter Regions, Genetic; Trans-Activators/*metabolism
Abstract :
[en] cAMP strongly stimulates the activity of the human prolactin (hPRL) promoter. We have previously shown that two types of cis-element are required for this cAMP regulation; binding sites for the pituitary-specific factor Pit-1, and the sequence spanning nucleotides -115 to -85 (named sequence A). Sequence A contains the TGACG motif found in the consensus sequence of the cAMP-responsive element (CRE). In this study, we show that a mutation in the TGACG motif of sequence A strongly reduces not only the cAMP regulation but also the Ca2+ regulation and basal activity of the hPRL promoter. Furthermore, gel-shift assays indicate that the mutation prevents binding of a ubiquitous factor which is not the CRE-binding protein. Southwestern experiments suggest that this ubiquitous factor's molecular mass is approximately 100 kDa. We conclude that binding of a 100-kDa ubiquitous factor to sequence A is required for full basal and hormonal regulation of hPRL-promoter activity.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Peers, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Nalda, Asunción M
Monget, Philippe
Voz, Marianne  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Belayew, Alexandra
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Binding of a 100-kDa ubiquitous factor to the human prolactin promoter is required for its basal and hormone-regulated activity
Publication date :
1992
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
210
Issue :
1
Pages :
53-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 August 2009

Statistics


Number of views
61 (2 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
31
Scopus citations®
without self-citations
24
OpenCitations
 
20

Bibliography


Similar publications



Contact ORBi