New Hosts of The Lassa Virus.

[en] Lassa virus (LASV) causes a deadly haemorrhagic fever in humans, killing several thousand people in West Africa annually. For 40 years, the Natal multimammate rat, Mastomys natalensis, has been assumed to be the sole host of LASV. We found evidence that LASV is also hosted by other rodent species: the African wood mouse Hylomyscus pamfi in Nigeria, and the Guinea multimammate mouse Mastomys erythroleucus in both Nigeria and Guinea. Virus strains from these animals were isolated in the BSL-4 laboratory and fully sequenced. Phylogenetic analyses of viral genes coding for glycoprotein, nucleoprotein, polymerase and matrix protein show that Lassa strains detected in M. erythroleucus belong to lineages III and IV. The strain from H. pamfi clusters close to lineage I (for S gene) and between II &III (for L gene). Discovery of new rodent hosts has implications for LASV evolution and its spread into new areas within West Africa.

Disciplines :

Immunology & infectious disease

Author, co-author :

Olayemi, Ayodeji

Cadar, Daniel

Magassouba, N. Faly

Obadare, Adeoba

Kourouma, Fode

Oyeyiola, Akinlabi

Fasogbon, Samuel

Igbokwe, Joseph

Rieger, Toni

Bockholt, Sabrina

More authors (10 more)

Language :

English

Title :

New Hosts of The Lassa Virus.

Publication date :

2016

Journal title :

Scientific Reports

eISSN :

2045-2322

Publisher :

Nature Publishing Group, London, United Kingdom

Volume :

Pages :

25280

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 June 2016

Statistics

Number of views

50 (1 by ULiège)

Number of downloads

1 (1 by ULiège)

More statistics

Scopus citations^®

119

Scopus citations^®
without self-citations

OpenCitations

100

Bibliography

McCormick, J. B. & Fisher-Hoch, S. P. Lassa fever. Curr. Top. Microbiol. Immunol. 262, 75-109 (2002).
Frame, J. D., Baldwin, J. M. J., Gocke, D. J. & Troup, J. M. Lassa fever, a new virus disease of man from West Africa. Am. J. Trop. Med. Hyg. 19, 670-676 (1970).
Monath, T. P., Newhouse, V. F., Kemp, G. E., Setzer, H. W. & Cacciapuoti, A. Lassa virus isolation from Mastomys natalensis rodents during an epidemic in Sierra Leone. Science 185, 263-265 (1974).
Wulff, H., Fabiyi, A. & Monath, T. P. Recent isolation of Lassa virus from Nigerian rodents. B. World Health Organ. 52, 609-613 (1975).
McCormick, J. B., Webb, P. A., Krebs, J. W., Johnson, K. M. & Smith, E. S. A prospective study of the epidemiology and ecology of Lassa fever. J. Infect. Dis. 155, 437-444 (1987).
Lecompte, E. et al. Mastomys natalensis and Lassa fever, West Africa. Emerg. Infect. Dis. 12, 1971-1974 (2006).
Leski, T. A. et al. Sequence variability and geographic distribution of Lassa virus, Sierra Leone. Emerg. Infect. Dis. 21, 609-618, doi: 10.3201/eid2104.141469 (2015).
Safronetz, D. et al. Geographic distribution and genetic characterization of Lassa virus in sub-Saharan Mali. PLos Neglected Tropical Diseases 7, e2582, doi: 10.1371/journal.pntd.0002582 (2013).
Kouadio, L. et al. Lassa Virus in Multimammate Rats, Cote d'Ivoire, 2013. Emerg. Infect. Dis. 21, 1481-1483, doi: 10.3201/eid2108.150312 (2015).
Nicolas, V., Olayemi, A., Wendelen, W. & Colyn, M. Mitochondrial DNA and morphometrical identification of a new species of Hylomyscus (Rodentia: Muridae) from West Africa. Zootaxa 2579, 30-44 (2010).
Olschlager, S. et al. Improved detection of Lassa virus by reverse transcription-PCR targeting the 5′ region of S RNA. J Clin Microbiol 48, 2009-2013 (2010).
Bowen, M. D. et al. Genetic diversity among Lassa virus strains. J. Virol. 74, 6992-7004 (2000).
Asogun, D. A. et al. Molecular diagnostics for lassa fever at Irrua specialist teaching hospital, Nigeria: lessons learnt from two years of laboratory operation. PLos Neglected Tropical Diseases 6, e1839, doi: 10.1371/journal.pntd.0001839 (2012).
Andersen, K. G. et al. Clinical Sequencing Uncovers Origins and Evolution of Lassa Virus. Cell 162, 738-750, doi: 10.1016/j.cell.2015.07.020 (2015).
Gonzalez, J. P. et al. An arenavirus isolated from wild-caught rodents (Praomys species) in the Central African Republic. Intervirology 19, 105-112 (1983).
Gryseels, S. et al. Gairo virus, a novel arenavirus of the widespread Mastomys natalensis: Genetically divergent, but ecologically similar to Lassa and Morogoro viruses. Virology 476, 249-256, doi: 10.1016/j.virol.2014.12.011 (2015).
Coulibaly-N'Golo, D. et al. Novel arenavirus sequences in Hylomyscus sp. and Mus (Nannomys) setulosus from Cote d'Ivoire: implications for evolution of arenaviruses in Africa. PLos ONE 6, e20893, doi: 10.1371/journal.pone.0020893 (2011).
Kronmann, K. C. et al. Two novel arenaviruses detected in pygmy mice, Ghana. Emerg. Infect. Dis. 19, 1832-1835, doi: 10.3201/eid1911.121491 (2013).
Fichet-Calvet, E. & Rogers, D. J. Risk maps of Lassa fever in West Africa. PLos Neglected Tropical Diseases 3, e388, doi: 10.1371/journal.pntd.0000388 (2009).
Ehichioya, D. U. et al. Current molecular epidemiology of Lassa virus in Nigeria. J. Clin. Microbiol. 49, 1157-1161, doi: 10.1128/JCM.01891-10 (2011).
Gunther, S. et al. Lassa fever encephalopathy: Lassa virus in cerebrospinal fluid but not in serum. J. Infect. Dis. 184, 345-349 (2001).
Lukashevich, L. S., Clegg, J. C. & Sidibe, K. Lassa virus activity in Guinea: distribution of human antiviral antibody defined using enzyme-linked immunosorbent assay with recombinant antigen. J. Med. Virol. 40, 210-217 (1993).
Fichet-Calvet, E., Becker-Ziaja, B., Koivogui, L. & Gunther, S. Lassa serology in natural populations of rodents and horizontal transmission. Vector Borne Zoonotic Dis. 14, 665-674, doi: 10.1089/vbz.2013.1484 (2014).
Fichet-Calvet, E. et al. Diversity and dynamics in a community of small mammals in coastal Guinea, West Africa. Belg. J. Zool. 139, 93-102 (2009).
Brouat, C., Loiseau, A., Kane, M., Ba, K. & Duplantier, J. M. Population genetic structure of two ecologically distinct multimammate rats: the commensal Mastomys natalensis and the wild Mastomys erythroleucus in southeastern Senegal. Mol Ecol 16, 2985-2997 (2007).
Irwin, N. R., Bayerlova, M., Missa, O. & Martinkova, N. Complex patterns of host switching in New World arenaviruses. Mol. Ecol. 21, 4137-4150, doi: 10.1111/j.1365-294X.2012.05663.x (2012).
Parrish, C. R. et al. Cross-species virus transmission and the emergence of new epidemic diseases. Microbiology and molecular biology reviews : MMBR 72, 457-470, doi: 10.1128/MMBR.00004-08 (2008).
Vieth, S. et al. RT-PCR assay for detection of Lassa virus and related Old World arenaviruses targeting the L gene. T. Roy. Soc. Trop. Med. H. 101, 1253-1264 (2007).
Jiang, H., Lei, R., Ding, S. W. & Zhu, S. Skewer: a fast and accurate adapter trimmer for next-generation sequencing paired-end reads. BMC bioinformatics 15, 182, doi: 10.1186/1471-2105-15-182 (2014).
Langmead, B. & Salzberg, S. L. Fast gapped-read alignment with Bowtie 2. Nature methods 9, 357-359, doi: 10.1038/nmeth.1923 (2012).
Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078-2079, doi: 10.1093/bioinformatics/btp352 (2009).
McKenna, A. et al. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome research 20, 1297-1303, doi: 10.1101/gr.107524.110 (2010).
Darriba, D., Taboada, G. L., Doallo, R. & Posada, D. jModelTest 2: more models, new heuristics and parallel computing. Nature methods 9, 772, doi: 10.1038/nmeth.2109 (2012).
Milne, I. et al. TOPALi v2: a rich graphical interface for evolutionary analyses of multiple alignments on HPC clusters and multi-core desktops. Bioinformatics 25, 126-127, doi: 10.1093/bioinformatics/btn575 (2009).
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30, 2725-2729, doi: 10.1093/molbev/mst197 (2013).
Martin, D. & Rybicki, E. RDP: detection of recombination amongst aligned sequences. Bioinformatics 16, 562-563 (2000).
Huson, D. H. & Bryant, D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol 23, 254-267, doi: 10.1093/molbev/msj030 (2006).